FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-146-5-7-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-146-5-7-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34223
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC838424.49814452268942No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT370510.82605265464746No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC28068.199164304707361No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG20505.990123601086989No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT13373.9067293925138067No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTT13223.862899219822926No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC8652.5275399585074365No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCT7302.1330684042895127No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC6821.9928118516786955No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCT5271.5399000672062648No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC3901.1395844899628904No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC3811.1132863863483622No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG3641.063612190632031No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3481.0168600064284252No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTT3030.8853694883557842No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCT2670.7801770738976712No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2390.698360751541361No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC2360.689594717003185No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC2320.6779066709522835No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2260.6603746018759314No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2010.5873243140577974No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTT1920.5610262104432692No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC1840.5376501183414663No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1670.4879759226251352No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTC1660.4850539111124098No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTT1600.4675218420360576No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCT1320.38570551967974753No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCG1320.38570551967974753No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCT1270.3710954621161207No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGC1260.3681734506033954No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGC1200.3506413815270432No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTT1170.3418753469888671No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1160.33895333547614176No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1140.3331093124506911No Hit
CCACGTCTAGAACAGGCTCCTCTAGTATCCATCGTTTACGGCTAGGACTA1120.32726528942524036No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTT1060.30973322034888817No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCCGTGGGGGTATCTAATCCCAG1000.292201151272536No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC990.2892791397598106No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT980.28635712824708526No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA970.2834351167343599No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAG900.2629810361452824No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTT860.251292990094381No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG850.2483709785816556No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTCG820.23960494404347954No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAA810.23668293253075418No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC810.23668293253075418No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC770.22499488647985275No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAGCAGGGGTATCTAATCCCAGTC740.21622885194167665No Hit
CCACGTCTAGAACAGGCTCCTCTAGGAGGGGTATCTAATCCCAGTCGCTT730.2133068404289513No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATC730.2133068404289513No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGGGGGTATCTAATCCCAGTCG710.2074628174035006No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGCGGGGTATCTAATCCCAGTCGCT690.20161879437804986No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA680.1986967828653245No Hit
CCACGTCTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTCGCTT660.19285275983987377No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT650.1899307483271484No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTCGCT650.1899307483271484No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTC640.18700873681442304No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGGTATCTAATCCCAGT630.1840867253016977No Hit
CCACGTCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT610.17824270227624697No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTC570.16655465622534554No Hit
CCACGTCTAGAACAGGCTCCTCTAGCACATGGGGTATCTAATCCCAGTCG570.16655465622534554No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGC550.16071063319989481No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT520.15194459866171872No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC510.14902258714899336No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCG510.14902258714899336No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC490.14317856412354263No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC490.14317856412354263No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT480.1402565526108173No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTC470.13733454109809193No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA460.13441252958536656No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTA460.13441252958536656No Hit
CCACGTCTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTC460.13441252958536656No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC440.12856850655991583No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT440.12856850655991583No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTCGGGGTATCTAATCCCAGTCGC430.1256464950471905No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT420.12272448353446512No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGCTCGGGGTATCTAATCC410.11980247202173977No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC390.11395844899628904No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTCGCT370.10811442597083833No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCG360.10519241445811298No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC360.10519241445811298No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC360.10519241445811298No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC350.10227040294538761No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG108.429218E-4295.05
GTTAGAA108.429218E-4295.05
CGTTAGA108.429218E-4295.04
CGTCTAG33300.0294.557074
CCACGTC33850.0293.692781
CACGTCT33700.0293.249272
GTCTAGA33450.0293.236185
ACGTCTA33700.0292.811583
TCTAGAA33700.0290.623176
CTAGAAC34000.0288.058847
AGAACAG35000.0278.985729
TAGAACA35450.0275.444278
CCACGTT353.8944563E-9210.714281
ACGTTAG150.002837672196.666673
CGTCTAA304.6048626E-7196.666674
CGTTCTA150.002837672196.666674
TTCTAGA200.0067093396147.56
ACGTTCT200.0067093396147.53
TTAGAAC200.0067093396147.56
GTCTAAA401.9296367E-6147.55
Produced by FastQC (version 0.11.5)