FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-145-5-1-3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-145-5-1-3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34491
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT683019.80226725812531No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTA362110.498390884578585No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCT23576.833666753645878No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT21516.236409498129946No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGA13053.7835957206227713No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTA12963.7575019570322694No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT10052.9138036009393757No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATC9302.6963555710185263No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCG6972.0208170247310893No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT6621.919341277434693No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC5301.536632744773999No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGAT5121.4844452175929952No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT5091.4757472963961613No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAAC4901.4206604621495462No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGA4271.2380041170160332No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCG3981.1539242121133049No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT3791.09883737786669No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTA3741.0843408425386332No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT3681.0669450001449654No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAA3671.064045693079354No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA3661.0611463860137427No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT2780.8060073642399466No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2390.6929343886811051No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAA2370.6871357745498826No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2250.6523440897625468No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG1860.5392711142037053No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG1440.4175002174480299No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1440.4175002174480299No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTA1360.39430576092313935No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCG1300.3769099185294715No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTAACCG1290.37401061146386017No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGA1260.3653126902670262No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTA1190.34501754080774694No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAA1190.34501754080774694No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTAAC1180.3421182337421356No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1100.3189237772172451No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACC1090.31602447015163376No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGA1020.2957293206923545No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACCGA980.2841320924299093No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAGTA930.26963555710185266No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT840.2435417935113508No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTAA830.2406424864457395No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT820.23774317938012815No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACC820.23774317938012815No Hit
GAGTGGTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTAA780.22614595111768288No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACCTTACATGTAAAAGGGGTATCTA770.22324664405207156No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT750.2174480299208489No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAACCG750.2174480299208489No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA740.2145487228552376No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTA740.2145487228552376No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC720.20875010872401495No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT690.200052187527181No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTAA610.17685773100229046No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGCTCGTGGGCTCGGAGGGGTATCT560.16236119567423385No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCCCGGGGTATCTAATC540.15656258154301123No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC530.1536632744773999No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAA520.1507639674117886No Hit
GAGTGGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTAACCG520.1507639674117886No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA500.14496535328056592No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTAAC500.14496535328056592No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA480.1391667391493433No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACC480.1391667391493433No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTAAC460.13336812501812068No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC440.12756951088689802No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGA430.1246702038212867No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTAACCG410.11887158969006408No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGTCGGGGTATCTAATCCCAGT400.11597228262445276No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAACC400.11597228262445276No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGAT390.11307297555884144No Hit
GAGTGGTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTAAC390.11307297555884144No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT360.10437505436200747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGG108.4294844E-4295.04
GAGTGGT34150.0293.704251
TGGTAGA33950.0293.262154
AGTGGTA34300.0292.419832
GGTAGAA33900.0291.954285
GTGGTAG34250.0291.554753
GAACAGG33300.0291.456459
TAGAACA35050.0279.429387
GTAGAAC35600.0277.1846
AGAACAG35350.0277.057988
GAACGGC304.6053356E-7196.666669
GGTAGAC304.6053356E-7196.666665
GAGTGGA255.5100987E-5177.01
AGAACGG503.2821845E-8147.58
GAAAAGG200.0067095505147.59
GGTAGGA200.0067095505147.55
TAGAACG805.412403E-792.18757
GACAGGC508.6982036E-488.59
AGACAGG550.001270078880.4545448
GAGTGTA650.002464305668.076931
Produced by FastQC (version 0.11.5)