FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-144-6-7-4-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-144-6-7-4-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33338
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC1999359.97060411542384No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC21636.488091667166597No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC14724.415381846541484No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC14694.406383106365109No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC7512.252684624152619No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC4351.3048173255744195No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC3631.0888475613414121No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC3571.0708500809886616No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC3110.9328693982842402No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC2620.7858899754034434No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTC2200.6599076129341892No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTCTGAAGCACCGCCAAGTCC2150.6449097126402303No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC1880.5639210510528526No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTC1810.5429239906413103No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC1490.44693742875997355No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC1240.37194792729017934No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTT1140.34195212670226166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTC1140.34195212670226166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTC1080.32395464634951104No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC1060.31795548623192754No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACCGTCTGGATCTTGCCATTGA860.25796388505609213No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGATATGAAGCACCGCCAGGTC740.2219689243505909No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAACACCGCCAGGTC710.2129701841742156No Hit
TTCTCAGCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC650.194972703821465No Hit
TTCTCAGCTAGAACAGGCTCCTCGGGCGGCTCGTATCCCAGTTTGGGTGA560.16797648329233908No Hit
TTCTCAGCTAGAACAGGCTCCTCTCTCGGCAACACCTTCTCCGGCCAGAG540.16197732317475552No Hit
TTCTCAGCTAGAACAGGCTCCTCTCCTTTCAAAGAGGATGCAGTATGGTT520.155978163057172No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTTGACCG490.14697942288079668No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTC480.14397984282200493No Hit
TTCTCAGCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC470.14098026276321315No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC450.13498110264562962No Hit
TTCTCAGCTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC440.13198152258683785No Hit
TTCTCAGCTAGAACAGGCTCCTCTCCCGGCAGGGCGAAGTCTATCCGAGT430.12898194252804607No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCAACGCCAGGTC390.11698362229287901No Hit
TTCTCAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC380.11398404223408724No Hit
TTCTCAGCTAGAACAGGCTCCTTTAGAGGGATATAAAGCACCGCCAAGTC380.11398404223408724No Hit
TTCTCAGCTAGAACAGGCTCCTCGCAGTTGTCCTCAGAGCGATTTACTGC370.11098446217529545No Hit
TTCTCGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC360.10798488211650369No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC350.10498530205771191No Hit
TTCTCAGCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC350.10498530205771191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAG32900.0295.05
CAATTTT108.4282213E-4295.0295
CTCAGCT33050.0294.553683
TCTCAGC33050.0294.553682
TCAGCTA33000.0294.5534
AGCTAGA32900.0294.551676
GCTAGAA32800.0294.550327
TTCTCAG33350.0293.673161
CTAGAAC33000.0291.424228
TAGAACA34150.0280.74679
CTCAGTA251.85506E-7236.03
TCTCAGT251.85506E-7236.02
CGCTGCG150.0028373373196.66667295
ACACAGG150.0028373373196.66667295
TCAGTAG304.6030436E-7196.666674
CTCACGA150.0028373373196.66667295
ACGGTAA150.0028373373196.66667295
CAGCACG200.0067085503147.5295
GAGGAGA200.0067085503147.5295
GCTAGAC200.0067085503147.57
Produced by FastQC (version 0.11.5)