FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-143-4-8-2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-143-4-8-2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34998
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC465013.28647351277216No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG444812.709297674152808No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC28388.109034801988685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT17334.951711526372936No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG15074.305960340590891No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC14964.274529973141322No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG9152.6144351105777472No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG8922.5487170695468313No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGT7882.2515572318418196No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG6831.9515400880050289No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA5931.6943825361449227No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT5841.668666780958912No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTT5631.6086633521915539No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA4811.3743642493856794No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC4471.2772158409051946No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG4271.2200697182696154No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4061.1600662895022573No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG3420.9771986970684038No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT2720.7771872678438768No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTG2620.7486142065260871No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT2190.625750042859592No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA2060.5886050631464655No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGT1970.5628893079604549No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG1960.5600320018286759No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA1930.5514600834333391No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1900.5428881650380022No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1770.5057431853248757No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC1740.4971712669295389No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGT1680.48002743013886506No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA1660.47431281787530716No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTG1570.4485970626892965No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1540.44002514429395967No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTGT1510.4314532258986228No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC1500.4285959197668438No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA1480.4228813075032859No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1460.417166695239728No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1400.4000228584490543No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT1350.38573632779015943No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1340.38287902165838045No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG1200.34287673581347505No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGT1150.32859020515458026No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT1130.32287559289102236No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTG1020.2914452254414538No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTTGGGGTATCTAATCCCAG1020.2914452254414538No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCT980.2800160009143379No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGT960.2743013886507801No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG920.2628721641236642No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAG920.2628721641236642No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTG890.2543002457283273No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT850.2428710212012115No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGACAGTGATTGACACTGGGGTAT840.24001371506943253No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGT830.23715640893765358No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC780.22286987827875876No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG780.22286987827875876No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC740.21144065375164295No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTG730.208583347619864No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC710.20286873535630606No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTG700.20001142922452714No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGT680.1942968169609692No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGTCGGAGGGGTATCT640.18286759243385337No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTGTG630.1800102863020744No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGT630.1800102863020744No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT620.17715298017029543No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG610.17429567403851648No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTG540.15429453111606378No Hit
TTCTCAGCTAGAACAGGCTCCTCCTGGGGTATCTAATCCCAGTGTGTTGC530.15143722498428483No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC520.14857991885250585No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGT510.1457226127207269No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTG510.1457226127207269No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGCTGTTATAAAATCACCATCATG510.1457226127207269No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGT510.1457226127207269No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAAT490.14000800045716896No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT480.13715069432539004No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG460.1314360820618321No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC430.12286416366649522No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGT420.12000685753471627No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTGTGT410.11714955140293731No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGTGGGGGTATCTAATCCCA410.11714955140293731No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT390.11143493913937938No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG380.10857763300760044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGT380.10857763300760044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTTTATGTCCTGGGGTGGGGTATC360.10286302074404251No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGCGGGGTATCTAATCCCAGTGT360.10286302074404251No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC360.10286302074404251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT34550.0294.57313
TTCTCAG34900.0294.154721
TCTCAGC34700.0294.149842
TCAGCTA34850.0291.614044
CTAGAAC35000.0290.78578
CAGCTAG34950.0290.77975
GCTAGAA35000.0290.36437
AGCTAGA35100.0289.537026
TAGAACA36600.0278.476789
GTTGATT5700.059.00000495-99
TCTGAGG2150.059.00000430-34
GTATTTT351.4246325E-859.00000495-99
CCCTCGA351.4246325E-859.00000425-29
CTGAGGC2150.059.00000430-34
TGGGTCT2150.059.00000425-29
ACTCTGG351.4246325E-859.00000475-79
ACGGGGT1800.059.00000425-29
AAAGTTT452.7284841E-1159.000004110-114
AGGCCCT351.4246325E-859.00000425-29
GCCCTCG351.4246325E-859.00000425-29
Produced by FastQC (version 0.11.5)