FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-142-5-7-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-142-5-7-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33299
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT828724.886633232229197No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC33109.940238445598967No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT25327.603831946905312No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT21796.543740052253821No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTC10643.1952911498843806No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC10393.120213820234842No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT9062.720802426499294No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT7112.13519925523289No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT6291.888945613982402No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC5491.6486981591038772No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTC5251.5766239226403196No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC5201.5616084567104118No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTT4341.3033424427159974No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCT3411.0240547764197123No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT3210.9639929127000811No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTG3190.9579867263281181No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT3150.9459743535841917No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2820.8468722784468002No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2560.7687918556112796No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCT2510.7537763896813718No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2460.738760923751464No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2270.6817021532178144No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCG2250.6756959668458512No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1610.4834980029430313No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1380.41442685966545545No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA1350.4054175801075107No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTT1280.3843959278056398No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCG1250.37538664824769513No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTC1240.37238355506171356No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT1220.36637736868975046No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1200.36037118231778736No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCG1140.34235262320189797No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAA1100.3303402504579717No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1060.31832787771404547No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1040.3123216913420824No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT1010.30331241178413765No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTC1010.30331241178413765No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC1000.30030931859815607No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTT950.2852938526682483No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCGC930.2792876662962852No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG880.2642722003663774No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT790.23724436169254332No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTCAGATGTGATAAGAGGGGTATCT790.23724436169254332No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTC770.23123817532058022No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCGC740.22222889576263552No Hit
GAGTGGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTCGCTT690.20721342983272772No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCG680.20421033664674615No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTC660.19820415027478303No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTCGC660.19820415027478303No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC650.19520105708880148No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGCGTCAGGGGTATCTAATCCC650.19520105708880148No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGC630.18919487071683833No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT600.18018559115889368No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAG590.1771824979729121No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA580.17417940478693053No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCT570.17117631160094898No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCG560.16817321841496743No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT560.16817321841496743No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTC550.16517012522898586No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTC540.16216703204300428No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT530.15916393885702274No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCT530.15916393885702274No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCGCTT530.15916393885702274No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTCGCTT530.15916393885702274No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTCG520.1561608456710412No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT510.1531577524850596No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCG490.1471515661130965No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT480.14414847292711494No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCGCT480.14414847292711494No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAAT480.14414847292711494No Hit
GAGTGGTAGAACAGGCTCCTCTTATACACAGGGGTATCTAATCCCAGTCG470.14114537974113336No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT460.13814228655515182No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGAGGGGTATCTA430.12913300699720712No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTCGC410.12312682062524401No Hit
GAGTGGTAGAACAGGCTCCTCTAAGTCGGGGTATCTAATCCCAGTCGCTT390.11712063425328087No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTCGC380.11411754106729931No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCCAGTTCGAATCGCGATTGTCGGA370.11111444788131776No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT350.10510826150935462No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGCTTGGGGTATCTAATCCCAGT350.10510826150935462No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT340.10210516832337307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGAA32750.0293.648835
GAGTGGT33100.0293.21751
TGGTAGA33000.0291.871224
AGTGGTA33300.0291.456452
GAACAGG33000.0290.97739
GTGGTAG33450.0288.385653
GTAGAAC33650.0285.794926
TAGAACA34600.0278.374277
AGAACAG34850.0276.377328
TATGGAA150.002837318196.66667295
TGTTAGA150.002837318196.666674
TGGTATA150.002837318196.666674
GTGTTAG200.006708505147.53
GTTAGAA200.006708505147.55
GTGGTAT200.006708505147.53
GTGGTAA200.006708505147.53
CAGGAGA403.580915E-4110.625295
CAGATCA700.003304689463.214283295
GGGGCAC1000.059.00000430-34
GTTTGTG900.059.00000460-64
Produced by FastQC (version 0.11.5)