FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-141-5-7-5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-141-5-7-5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33121
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC827824.993206726850033No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG32319.75514024335014No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC27268.230427825246823No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT18375.546330122882763No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT11593.499290480359892No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC8662.6146553546088582No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC7712.327828266054769No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG6311.9051357145013739No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTT6311.9051357145013739No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTC5601.6907702062135803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA5601.6907702062135803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG4861.4673470003925002No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGC4861.4673470003925002No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT4651.403943117659491No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA4121.24392379457142No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC3421.0325775187947224No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC3401.026539053772531No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC3020.9118082183508952No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA2880.8695389631955557No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG2730.8242504755291206No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2730.8242504755291206No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCT2470.7457504302406328No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC1970.5947888046858488No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCT1870.564596479574892No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT1640.4951541318196914No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG1620.48911566679750007No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1610.4860964342864044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCG1560.471000271730926No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGC1490.44986564415325625No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1480.4468464116421606No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1380.4166540865312038No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG1360.41061562150901243No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1340.404577156486821No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC1090.32909634370942903No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT1070.32305787868723773No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT1060.320038646176142No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTT950.28682708855408956No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCT930.2807886235318982No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT890.26871169348751545No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC860.25965399595422844No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGC850.25663476344313274No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCG840.2536155309320371No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC830.2505962984209414No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC800.24153860088765433No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC800.24153860088765433No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCG800.24153860088765433No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT780.235500135865463No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC760.22946167084327163No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAG690.2083270432656019No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCG680.20530781075450621No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC620.18719241568793213No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTC610.18417318317683645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC600.18115395066574078No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCG600.18115395066574078No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTCG580.17511548564354942No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA570.17209625313245372No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGCCGGGGTATCTAATCCCAG570.17209625313245372No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTTGGGGTATCTAATCCCAGTCGC540.1630385555991667No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGGGTATCTAATCCCAGTC530.160019323088071No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGGGTATCTAATCCCAGTCG530.160019323088071No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCG520.15700009057697534No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT470.14190392802149693No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT470.14190392802149693No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG440.1328462304882099No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT420.12680776546601855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTC420.12680776546601855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCA420.12680776546601855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG390.1177500679327315No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTC380.11473083542163581No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT380.11473083542163581No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCG370.11171160291054015No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC370.11171160291054015No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTC350.10567313788834877No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAA340.10265390537725311No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG108.427992E-4295.04
CTCGCTA108.427992E-4295.03
CTCAGCT32550.0295.03
TTCTCGC108.427992E-4295.01
TTCTCAG32750.0295.01
TCTCAGC32700.0294.548922
TCAGCTA32400.0294.08954
GCTAGAA32200.0293.625767
CAGCTAG32300.0293.173375
AGCTAGA32300.0293.173376
CTAGAAC32450.0292.272748
TAGAACA33550.0282.25049
GCTGAAC304.6026253E-7196.666677
TCTCGCT150.0028372605196.666672
CACCAGG150.0028372605196.66667295
CTCAACT255.5081517E-5177.03
TCAGCAG200.0067083687147.54
GGAACAG200.0067083687147.5295
AGCTGAA453.4658642E-6131.111116
CAGCTAA453.4658642E-6131.111115
Produced by FastQC (version 0.11.5)