FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-140-6-7-4-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-140-6-7-4-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32216
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1996461.96920784703253No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT20156.2546560715172586No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT9292.8836602930221007No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT9112.8277874348149985No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT8672.6912093369754158No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT6762.0983362304444997No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT6001.8624286069034022No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT5791.7972436056617829No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT2250.6984107275887758No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT1720.5338962006456419No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGATATAAGCAGCATCAAATCCTT1650.5121678668984356No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGTACCGCCAGGTCCT1600.49664762850757393No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCCT1540.47802334243853983No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT1530.47491929476036754No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT1390.4314626272659548No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT1330.4128383411969208No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA1310.4066302458405761No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT1120.3476533399553017No Hit
TGCTTATAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTTC1010.31350881549540605No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT1000.3104047678172337No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCGAGTCCT790.24521976657561462No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTTGACC640.19865905140302956No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT600.1862428606903402No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT590.18313881301216786No Hit
TGCTTATAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT490.1520983362304445No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT470.14589024087409982No Hit
TGCTTATAGAACAGGCTCCTCGGGCGGCTCGTATCCCAGTTTGGGTGACC470.14589024087409982No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT450.13968214551775515No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCT440.1365780978395828No Hit
TGCTTATAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT420.13037000248323816No Hit
TGCTTATGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT410.1272659548050658No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTT390.12105785944872113No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT360.11174571641420412No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT360.11174571641420412No Hit
TGCTTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT350.1086416687360318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG31700.0295.03
CAGATGG108.4269274E-4295.0295
GCTTAAG108.4269274E-4295.02
CTTATGA157.175322E-6294.999973
GAAAGGC157.175322E-6294.999979
TGCTTAT32050.0294.53981
GCTTATA31850.0294.53692
TTATAGA31750.0294.535434
GAACAGG31500.0292.658729
TATAGAA31950.0291.76845
TAGAACA32750.0283.29017
ATAGAAC32800.0282.858226
AGAACAG32800.0281.958838
TTATGAA202.2616547E-5221.254
AGAAAGG202.2616547E-5221.258
GCTTATG202.2616547E-5221.252
CTTAAGA150.0028369033196.666663
AATCGAG150.0028369033196.66666295
TGCTTAA255.506764E-5177.01
TTAAGAA200.006707526147.54
Produced by FastQC (version 0.11.5)