FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-14-4-8-2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-14-4-8-2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11975
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT227919.031315240083508No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG136011.356993736951983No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG8246.881002087682672No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT6715.603340292275575No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG6535.453027139874739No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG3663.056367432150313No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA3032.53027139874739No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT2211.8455114822546974No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2161.803757828810021No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2051.7118997912317326No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT1951.62839248434238No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG1831.5281837160751566No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG1611.3444676409185805No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1591.3277661795407099No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1491.244258872651357No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTT1421.1858037578288099No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG1331.1106471816283925No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1291.0772442588726514No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1231.0271398747390397No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGC1110.9269311064718164No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1020.8517745302713988No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTGTGT730.6096033402922756No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT690.5762004175365345No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT680.5678496868475992No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTT640.5344467640918581No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTT630.5260960334029228No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT600.5010438413361169No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT580.4843423799582463No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTTG560.4676409185803758No Hit
GAGACTTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG540.4509394572025052No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT480.40083507306889354No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC480.40083507306889354No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT470.3924843423799582No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT450.3757828810020877No Hit
GAGACTTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTG440.3674321503131524No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGC420.3507306889352818No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTG410.34237995824634654No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG410.34237995824634654No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT400.33402922755741127No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG390.325678496868476No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT390.325678496868476No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTG390.325678496868476No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT380.3173277661795407No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTAGATGTGTATAAGAGGGGTATCT370.3089770354906054No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA360.3006263048016702No Hit
GAGACTTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTG290.24217118997912315No Hit
GAGACTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC290.24217118997912315No Hit
GAGACTTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTGTT290.24217118997912315No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT270.2254697286012526No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT260.2171189979123173No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTGT260.2171189979123173No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTGT260.2171189979123173No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGT250.20876826722338201No Hit
GAGACTTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTGTTGC240.20041753653444677No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTGT220.1837160751565762No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA210.1753653444676409No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT210.1753653444676409No Hit
GAGACTTAGAACAGGCTCCTCTTATAGGGTATCTAATCCCAGTGTGTTGC210.1753653444676409No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTTG200.16701461377870563No Hit
GAGACTTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTGTT200.16701461377870563No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC200.16701461377870563No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTTG190.15866388308977036No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT190.15866388308977036No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTG180.1503131524008351No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT180.1503131524008351No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTAGGGGTATCTAATC170.1419624217118998No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGT160.13361169102296452No Hit
GAGACTTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTGTG150.12526096033402923No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT150.12526096033402923No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGCGTGTTGCTG150.12526096033402923No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTGTT140.11691022964509395No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGCG130.10855949895615866No Hit
GAGACTTAGAACAGGCTCCTCTATGGGTATCTAATCCCAGTGTGTTGCTG130.10855949895615866No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT130.10855949895615866No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTGT130.10855949895615866No Hit
GAGACTTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTGTG120.10020876826722339No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT120.10020876826722339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTA11850.0295.02
GAGACTT11900.0295.01
GACTTAG11900.0293.76053
CTTAGAA11950.0292.53145
ACTTAGA12000.0291.31254
GAACAGG11800.0291.259
TTAGAAC12000.0287.6256
TAGAACA12150.0282.860087
AGAACAG12300.0278.21148
TGTTGAT2500.059.00000490-94
TTGTTGA2500.059.00000490-94
TGTTGAG2500.059.000004105-109
GGGTTTT900.059.00000450-54
GGGTTTA257.744082E-659.000004110-114
GTTGAGG2500.059.000004105-109
GAGCGGT257.744082E-659.00000485-89
TTGTATA452.5465852E-1159.00000490-94
GCGGTTT257.744082E-659.00000485-89
GGTTTTA900.059.00000450-54
CAATTGT452.5465852E-1159.00000490-94
Produced by FastQC (version 0.11.5)