FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-139-4-1-3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-139-4-1-3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33246
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTA457513.761053961378813No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCT335710.097455332972388No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT29258.798050893340552No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGA17645.305901461829995No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTA17585.287854177946219No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT11563.4771100282740783No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATC10893.275582024905252No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT9952.9928412440594356No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCG8612.589785237321783No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGAT7102.1355952595801No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAAC6111.8378150754978042No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCG5901.7746495819045902No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT5351.6092161463033148No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGA5171.5550742946519882No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTA5081.528003368826325No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT4981.4979245623533657No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA4791.4407748300547434No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAA4781.4377669494074474No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3270.9835769716657642No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAA3140.9444745232509174No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2680.8061120134753053No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG2530.7609938037658666No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT2150.6466943391686217No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTA2090.6286470552848463No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG1940.5835288455754076No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTA1910.5745052036335199No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTAACCG1890.568489442338928No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGA1600.48126090356734647No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAA1500.4511820970943873No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGA1420.42711905191601995No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTAAC1420.42711905191601995No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACC1370.4120796486795404No Hit
GAGTGGTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTAA1210.36395355832280574No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACCGA1170.3519220357336221No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCG1170.3519220357336221No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1150.34590627443903027No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1150.34590627443903027No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT1140.34289839379173437No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1100.3308668712025507No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT1060.31883534861336704No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAGTA1050.3158274679660711No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACC1020.3068038260241834No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTAA1010.3037959453768874No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1000.3007880647295915No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1000.3007880647295915No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAA880.26469349696204053No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACC830.24965409372556097No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAACCG820.24664621307826504No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA800.24063045178367323No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACCTTACATGTAAAAGGGGTATCTA770.23160680984178547No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC760.22859892919448957No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTAA740.22258316789989774No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC700.21055164531071408No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGA690.20754376466341815No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGTCGGGGTATCTAATCCCAGT680.20453588401612224No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTA680.20453588401612224No Hit
GAGTGGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTAACCG620.18648860013234675No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTAAC600.18047283883775492No Hit
GAGTGGTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTAAC600.18047283883775492No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCG570.17144919689586718No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTAAC570.17144919689586718No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAACC470.14137039042290803No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGAT460.1383625097756121No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAACC460.1383625097756121No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCCCGGGGTATCTAATC440.13234674848102027No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTAAC430.12933886783372434No Hit
GAGTGGTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTAACC430.12933886783372434No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAACC420.12633098718642843No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT410.12332310653913252No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT410.12332310653913252No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGCTCGTGGGCTCGGAGGGGTATCT380.11429946459724478No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTAACCG380.11429946459724478No Hit
GAGTGGTAGAACAGGCTCCTCTTATACACATCTGGGGTATCTAATCCCAG340.10226794200806112No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT340.10226794200806112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA32750.0295.000034
GAGTGGT32800.0295.01
AGTGGTA32900.0294.103332
GGTAGAA32850.0293.652955
GTGGTAG33150.0291.885353
GAACAGG33200.0290.112989
TAGAACA34350.0281.25917
AGAACAG34600.0279.653178
GTAGAAC35050.0274.80036
TGGAGAA200.0067084604147.499984
TGCTAGA200.0067084604147.499984
TTTACGG1250.059.000004110-114
TCCCTGG1250.059.00000475-79
GGCCAGG452.7284841E-1159.000004120-124
TAGGGCC452.7284841E-1159.000004115-119
CTACCGG900.059.00000425-29
AGTTCCC1250.059.00000470-74
CCTGGCG1250.059.00000475-79
GCCAGGC452.7284841E-1159.000004120-124
GGGATGT452.7284841E-1159.00000425-29
Produced by FastQC (version 0.11.5)