FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-138-5-2-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-138-5-2-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31530
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC913628.9755788138281No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC30129.55280685061846No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG20496.498572787821123No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG17305.486837932128132No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC16705.296542974944497No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC9312.952743418966064No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC7902.5055502695845226No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG7102.251823660006343No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC4731.5001585791309864No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC4401.3954963526799873No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT4151.3162067871868062No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3721.1798287345385348No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC3601.141769743101808No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG3571.132254995242626No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3291.0434506818902634No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG2890.9165873771011736No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA2740.8690136378052647No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2670.846812559467174No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT2380.754836663495084No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2170.6882334284808119No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1900.6026006977481764No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGT1810.5740564541706312No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTG1450.45987947986045036No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA1420.4503647320012686No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC1300.41230574056454167No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC1210.3837614969869965No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTG1160.3679035838883603No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1140.36156041864890576No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC1090.3457025055502696No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1080.3425309229305423No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG1060.33618775769108783No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGCTCGGGGTATCTAATCCCAGTT1010.3203298445924516No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAG940.2981287662543609No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC830.26324135743736127No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC820.260069774817634No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG780.24738344433872503No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC770.2442118617189978No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC710.2251823660006343No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA710.2251823660006343No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTAGGGGTATCTAATCCCA690.21883920076117983No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTG690.21883920076117983No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTG660.20932445290199808No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC660.20932445290199808No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT650.20615287028227083No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC630.19980970504281637No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA620.19663812242308915No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG610.19346653980336187No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT600.19029495718363465No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC580.18395179194418015No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG560.17760862670472566No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA560.17760862670472566No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGTGATTTGAGCCGATTGTGGAAC540.17126546146527116No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTG530.16809387884554391No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC520.1649222962258167No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT520.1649222962258167No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC490.15540754836663495No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC440.13954963526799874No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCTCGTGGGGTATCTAATCCCAG440.13954963526799874No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT420.13320647002854424No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT410.130034887408817No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT400.12686330478908975No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG390.12369172216936251No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGT380.12052013954963527No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGT370.11734855692990803No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC360.11417697431018078No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCCGTGGGCTCGGGGTATCTAAT360.11417697431018078No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC360.11417697431018078No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTTGGGGTATCTAAT350.11100539169045354No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGCTACAGGGGTATCTAATCCCA330.10466222645099904No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC320.1014906438312718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATC31150.0294.52652
TCAATCT31000.0294.048373
AATCTAG30900.0293.09065
TCTCAAT31500.0292.19051
ATCTAGA31050.0291.674746
TCTAGAA30800.0291.647747
CAATCTA31200.0290.74524
CTAGAAC31100.0288.83448
TAGAACA32200.0278.051249
AGACCGA150.0028366111196.66666295
CAATCTG255.505631E-5177.04
CTAGACC200.006706837147.58
GCACGAG200.006706837147.5295
CTAGACA550.001269060380.4545448
TAGAACG550.001269060380.4545449
CTGACAA550.001269060380.454544295
CAGCAGA600.001792535273.74999295
ATCACCA600.001792535273.74999295
TGGCGAA950.059.00000480-84
TCAATCA750.004341121859.0000043
Produced by FastQC (version 0.11.5)