FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-137-6-7-4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-137-6-7-4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30144
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1876062.23460721868365No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT13574.501725053078556No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT11873.9377653927813165No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT11673.8714171974522293No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT8462.8065286624203822No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT6482.1496815286624202No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT5111.695196390658174No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3481.1544585987261147No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT2560.8492569002123143No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2540.8426220806794055No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA2150.7132430997876857No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT2000.6634819532908706No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT1750.5805467091295117No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCT1600.5307855626326964No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGACACCGCCAAGTCCT1160.3848195329087049No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT1010.3350583864118896No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCACCAGGTCCT880.291932059447983No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT830.27534501061571126No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT780.25875796178343946No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTACT740.24548832271762208No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGCCTGAGGCACCGCCAAGTCCT640.21231422505307856No Hit
GAGTGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT510.16918789808917198No Hit
GAGTGGTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT460.15260084925690023No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTTGACCGAC420.1393312101910828No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTTGACC410.13601380042462843No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCAT400.1326963906581741No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT390.12937898089171973No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCT370.12274416135881104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGT30100.0295.01
GAACGGC108.424196E-4295.09
CAAACCG108.424196E-4295.0295
ACAACGG108.424196E-4295.0295
AGTGGTA30050.0294.509162
TGGTAGA29900.0294.506684
GGTAGAA29850.0294.011725
GAACAGG29650.0294.005079
GTAGAAC30000.0292.056
GTGGTAG30350.0290.143
TAGAACA30500.0285.811467
AGAACAG30950.0281.17938
GAAGAGG150.002835986196.66666295
GGTAGAC150.002835986196.666665
GTGGTAA150.002835986196.666663
AACGTCA200.0067053623147.5295
AGTGGTG200.0067053623147.52
GAATAGG200.0067053623147.5295
GGTAAAA200.0067053623147.55
GACGAAA550.001268507180.454544295
Produced by FastQC (version 0.11.5)