FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-136-4-7-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-136-4-7-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT611719.73416782269252No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG425413.72390876536439No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT21276.861954382682195No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC19436.268348549859663No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCG9212.9712552827693006No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTCT8452.726070264864342No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTTC7462.406684517856567No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTCT7432.397006161886634No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTTC6432.074394296222215No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC6372.0550375842823496No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT6181.9937413298061102No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT6141.9808368551795335No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCTG4631.4936929380262607No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTGT4311.3904571410136464No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2670.8613736813239992No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT2540.8194341387876246No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCGC2300.742007291028164No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTTC2290.7387811723715199No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCGC2140.6903893925218569No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC2140.6903893925218569No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT2100.6774849178952802No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT2020.6516759686421266No Hit
AGGAATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTCG1840.5936058328225312No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1660.5355356970029358No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG1500.48391779849662875No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCG1370.4419782559602542No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG1370.4419782559602542No Hit
AGGAATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCGCT1340.43229989999032165No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1330.42907378133367746No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCGCTT1310.4226215440203891No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1230.39681259476723557No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC1080.3484208149175727No Hit
AGGAATAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTCGCTTC1040.33551634029099586No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCG940.30325515372455397No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTCT920.2968029164112656No Hit
AGGAATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTCG920.2968029164112656No Hit
AGGAATAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG890.28712456044133305No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT890.28712456044133305No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCTT850.27422008581475626No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTCT840.2709939671581121No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT830.2677678485014679No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTC830.2677678485014679No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCGC780.2516372552182469No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCGCT760.24518501790495853No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGCT760.24518501790495853No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCTT730.23550666193502598No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTC720.23228054327838177No Hit
AGGAATAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCTTC700.22582830596509337No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTCG670.21614994999516082No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCGCTT630.20324547536858406No Hit
AGGAATAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTCGC610.19679323805529567No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTTC560.18066264477207472No Hit
AGGAATAGAACAGGCTCCTCTAGGTCACGTGGGCTCGGGGTATCTAATCC560.18066264477207472No Hit
AGGAAGGACTACCGGGGTATCTAATCCCAGTCGCTTCTGTCTCTTATACA540.17421040745878635No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCGC540.17421040745878635No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCGC510.16453205148885378No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGCTCGGGGTATCTAATCCCAGTCG510.16453205148885378No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT500.16130593283220956No Hit
AGGAATAGAACAGGCTCCTCTAGATTTGGGGTATCTAATCCCAGTCGCTT500.16130593283220956No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCGCT480.1548536955189212No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT470.15162757686227699No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT460.1484014582056328No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCGCTT460.1484014582056328No Hit
AGGAAGGACTACTGGGGTATCTAATCCCAGTCGCTTCTGTCTCTTATACA450.14517533954898862No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT450.14517533954898862No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCGTGGGGTATCTAAT440.1419492208923444No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTCGCTTCTG430.13872310223570022No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGGTATCTAATCCCAGTC410.13227086492241183No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT400.12904474626576767No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG380.12259250895247926No Hit
AGGAATAGAACAGGCTCCTCTATGTTGGGGTATCTAATCCCAGTCGCTTC370.11936639029583508No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCGC360.11614027163919088No Hit
AGGAATAGAACAGGCTCCTCTATGTCTGGGGGTATCTAATCCCAGTCGCT350.11291415298254669No Hit
AGGAATAGAACAGGCTCCTCTAGATGTGTATAAGAGACAGGGGTATCTAA340.10968803432590252No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA340.10968803432590252No Hit
AGGAATAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTCGCTT340.10968803432590252No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATCTAA330.1064619156692583No Hit
AGGAATAGAACAGGCTCCTCTAGGATGTGTATAAGAGACAGCATAGTGGG330.1064619156692583No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCTT330.1064619156692583No Hit
AGGAATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTCGCTTC310.10000967835596994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAG30200.0294.023162
AGGAATA30600.0292.107851
GAACAGG30250.0290.611578
AACAGGC30300.0289.158429
GAATAGA30800.0288.295443
ATAGAAC31500.0280.015875
AGAACAG31500.0279.079387
AATAGAA32250.0274.41864
TAGAACA32100.0273.40346
GGAAGGA301.546141E-9245.833342
AGAACGG202.260807E-5221.257
GAACGGC202.260807E-5221.258
AGGAAGG353.8853614E-9210.714281
AGGAGGA255.504703E-5177.0295
AACGGCT255.504703E-5177.09
GGAATAA359.909909E-7168.571432
AAGGACT451.7451384E-8163.88894
GAAGGAC451.7451384E-8163.88893
CTACTGG200.0067062736147.59
CTACCGG301.1383856E-4147.59
Produced by FastQC (version 0.11.5)