FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-135-4-7-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-135-4-7-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30810
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC432414.03440441415125No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT399712.973060694579683No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT30139.779292437520287No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTC15585.056799740344045No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC15334.975657254138267No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT12534.06686140863356No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT10283.336579032781564No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC7892.5608568646543333No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTC6832.216812723141837No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTT6091.976630963972736No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTG4951.6066212268743916No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCT4591.489776046738072No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT4361.415124959428757No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT3841.2463485881207401No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT3621.1749432002596558No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA3481.1295034079844206No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3391.100292112950341No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCG3391.100292112950341No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCT3000.9737098344693282No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT2100.6815968841285297No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1930.6264199935086011No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCG1710.555014605647517No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTT1660.5387861084063615No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTC1620.5258033106134372No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCG1620.5258033106134372No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAA1600.519311911716975No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1500.4868549172346641No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1350.4381694255111977No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCGC1310.42518662771827326No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG1290.4186952288218111No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1270.4122038299253489No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1250.4057124310288867No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT1230.39922103213242455No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTT1140.37000973709834467No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTC1140.37000973709834467No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGC1110.36027263875365145No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCGC1110.36027263875365145No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTCAGATGTGATAAGAGGGGTATCT1100.35702693930542034No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1070.347289840960727No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT1050.34079844206426485No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC1050.34079844206426485No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTCGC1020.3310613437195716No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGCGTCAGGGGTATCTAATCCC980.3180785459266472No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC970.3148328464784161No Hit
GAGTGGTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTCGCTT930.30185004868549176No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCT900.2921129503407984No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAG850.275884453099643No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT830.26939305420318077No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCG810.2629016553067186No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTC810.2629016553067186No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTC790.2564102564102564No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTCGC770.24991885751379422No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCG770.24991885751379422No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACGGGGTATCTAATCCCAGTCGCTT750.24342745861733206No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTC720.23369036027263873No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCT710.23044466082440765No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT680.2207075624797144No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT660.2142161635832522No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTCG640.20772476468679No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCGCT630.2044790652385589No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCG610.19798766634209672No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTGGGGGTATCTAATCCCAGTCGCT580.18825056799740345No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT570.18500486854917234No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCGCTT570.18500486854917234No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTC530.17202207075624798No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGCTTGGGGTATCTAATCCCAGT520.16877637130801687No Hit
GAGTGGTAGAACAGGCTCCTCTTATACACAGGGGTATCTAATCCCAGTCG510.1655306718597858No Hit
GAGTGGTAGAACAGGCTCCTCTAAGTCGGGGTATCTAATCCCAGTCGCTT500.16228497241155468No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCCAGTTCGAATCGCGATTGTCGGA480.15579357351509251No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTCGCTTC480.15579357351509251No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTCGC470.1525478740668614No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT460.1493021746186303No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT420.13631937682570594No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCGCT400.12982797792924375No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA390.12658227848101267No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA370.12009087958455046No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCTAA350.11359948068808828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGT30550.0294.517181
AGTGGTA30500.0294.032782
GGTAGAA30450.0293.062385
TGGTAGA30550.0292.58594
GTGGTAG30600.0292.107823
GAACAGG30450.0291.60929
GTAGAAC30600.0290.179726
TAGAACA32300.0276.27717
AGAACAG32400.0275.424388
AGTGGTG150.0028363054196.666672
GAAAGGC352.103231E-4126.4285669
AGAAAGG650.002461814668.076928
GGCACCG1550.059.00000435-39
TCTAGGC900.059.00000420-24
TCAGGGG452.7284841E-1159.00000425-29
CCTTTGG1250.059.00000445-49
CTCTAGG20150.059.00000415-19
GGGGCAC351.4231773E-859.030-34
CTTGTGG201.8424298E-459.025-29
TTCTATC1100.059.0100-104
Produced by FastQC (version 0.11.5)