FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-134-5-7-5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-134-5-7-5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30200
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC965431.966887417218544No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC327010.827814569536425No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT21727.19205298013245No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG16415.433774834437086No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTT10233.3874172185430464No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC9953.2947019867549674No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC7272.4072847682119205No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4911.6258278145695364No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC3951.3079470198675498No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTT3871.281456953642384No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3741.23841059602649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCT3521.1655629139072847No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCT3501.1589403973509933No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC3071.0165562913907285No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2830.9370860927152318No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2790.923841059602649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2200.728476821192053No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2150.7119205298013245No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTC2040.6754966887417219No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCT2030.6721854304635762No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCG1790.5927152317880794No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1730.5728476821192053No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1430.47350993377483447No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1340.4437086092715232No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCT1290.42715231788079466No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC1230.4072847682119205No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG1190.39403973509933776No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1080.3576158940397351No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1070.35430463576158944No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGC930.30794701986754963No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT900.2980132450331126No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG830.27483443708609273No Hit
CGATTCCTAGAACAGGCTCCTCTATGCGTCAGGGGTATCTAATCCCAGTC740.2450331125827815No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC720.23841059602649006No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT710.23509933774834438No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC690.22847682119205298No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT650.2152317880794702No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC640.2119205298013245No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTT590.19536423841059603No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGT580.19205298013245034No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT570.18874172185430466No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT570.18874172185430466No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGT550.18211920529801326No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTC540.17880794701986755No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC510.16887417218543047No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTC470.1556291390728477No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGC460.152317880794702No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC450.1490066225165563No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTC440.1456953642384106No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA440.1456953642384106No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCGCT430.1423841059602649No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA430.1423841059602649No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTC430.1423841059602649No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC420.1390728476821192No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC400.13245033112582782No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTC390.12913907284768214No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTGGGGTATCTAATCCCAGTCGC380.12582781456953643No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC380.12582781456953643No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTC380.12582781456953643No Hit
CGATTCCTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTCG350.11589403973509935No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATC330.10927152317880796No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCG330.10927152317880796No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGT310.10264900662251657No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC310.10264900662251657No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCT310.10264900662251657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACGG157.170902E-6295.09
ATTCTAG255.18412E-10295.03
CCTAGAA29400.0295.06
CGATTCC29750.0295.01
GATTCCT29800.0294.505042
TCCTAGA29650.0294.502535
TTCCTAG29650.0294.502534
ATTCCTA29850.0293.517583
CTAGAAC29850.0290.058627
TAGAACA30500.0281.4598
AGAACAG30900.0277.815559
GATTCTA301.544322E-9245.833342
TTCTAGA301.544322E-9245.833344
CGATTCT353.8835424E-9210.714281
CCTAGAT150.002836032196.666676
CCTAGAC150.002836032196.666676
TCTAGAA408.627467E-9184.3755
TAGAACG453.460882E-6131.111118
GCCCTCG150.004373151359.00000425-29
GGGTTTG600.059.00000425-29
Produced by FastQC (version 0.11.5)