FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-133-4-7-5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-133-4-7-5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30566
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG447614.643721782372571No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC389712.749460184518746No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT26318.607603219263234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT16055.250932408558529No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC12554.105869266505267No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTT8932.921546816724465No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG8072.6401884446770922No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTC7982.6107439638814367No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA7862.5714846561538964No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT7362.407904207289145No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG6852.241052149447098No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGC6422.1003729634234114No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC4611.5082117385330105No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA4251.3904338153503892No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC4221.3806189884185043No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4181.367532552509324No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG3801.2432114113721127No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCT3271.069816135575476No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT2640.863704770005889No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT2530.8277170712556436No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCT2480.8113590263691683No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGC2380.778642936596218No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG2360.772099718641628No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC2330.7622848917097428No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG2030.6641366223908919No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1840.6019760518222862No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCT1680.5496303081855657No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1620.5300006543217954No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCG1620.5300006543217954No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1560.5103710004580253No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1560.5103710004580253No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC1530.5005561735261401No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT1510.4940129555715501No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT1260.41222273113917424No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1210.39586468625269905No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTT1180.386049859320814No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCG1100.3598769875024537No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCG1070.3500621605705686No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT1030.3369757246613885No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGC1020.33370411568409347No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAG990.32388928875220835No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA940.30753124386573316No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT940.30753124386573316No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA880.28790159000196297No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTCG860.2813583720473729No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCG830.2715435451154878No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTC810.2650003271608977No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCA780.25518550022901265No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGGGTATCTAATCCCAGTC760.24864228227442253No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC710.2322842373879474No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCG710.2322842373879474No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCG710.2322842373879474No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC700.22901262841065234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGCCGGGGTATCTAATCCCAG690.2257410194333573No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTTGGGGTATCTAATCCCAGTCGC660.21592619250147224No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT620.2028397565922921No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGGGTATCTAATCCCAGTCG620.2028397565922921No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCT540.17666688477393183No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT540.17666688477393183No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAA510.16685205784204674No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG510.16685205784204674No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG490.16030883988745664No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCG480.15703723091016164No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC460.15049401295557155No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG440.14395079500098149No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTC430.14067918602368645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT390.12759275011450633No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTC370.12104953215991625No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT370.12104953215991625No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC350.11450631420532617No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC340.11123470522803114No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACACACACACACACACACACATGG340.11123470522803114No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC330.10796309625073612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCAG30250.0295.01
CTCAGCT30250.0294.024783
TCTCAGC30350.0293.5422
TCAGCTA30350.0292.574
CAGCTAG30250.0292.074375
AGCTAGA30250.0292.074376
CTAGAAC30050.0292.05498
GCTAGAA30100.0291.569767
TAGAACA31800.0276.446539
GCTAGAC453.4615332E-6131.111117
TTGATTT4250.059.00000495-99
CCAGGTC4300.059.00000440-44
TCTAGGC1300.059.00000420-24
GGGACTA1250.059.00000425-29
AGGTTTA5000.059.000004110-114
TGATTTA4250.059.00000495-99
TCTCGTG6150.059.00000425-29
TAGGGAC1250.059.00000420-24
GCCAGGT4300.059.00000440-44
CTCTAGG20000.059.00000420-24
Produced by FastQC (version 0.11.5)