FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-132-5-5-7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-132-5-5-7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29762
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC588219.76345675693838No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG373712.556279819904576No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG20306.820778173509845No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC15935.352462872118809No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC14104.7375848397285125No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG7312.456152140313151No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT6532.1940729789664672No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC5581.8748740004031987No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG5551.8647940326590955No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA5501.8479940864189235No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT5381.8076742154425105No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4371.4683153013910355No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA4251.4279954304146227No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATC3941.323835763725556No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3501.1759962368120422No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCC3461.1625562798199045No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3461.1625562798199045No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG2970.9979168066662186No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCAT2760.9273570324574961No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC2590.8702372152409112No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC2540.8534372690007391No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1950.6551979033667092No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCAT1940.6518379141186749No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1720.5779181506619179No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1660.5577582151737115No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCA1640.5510382366776426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1580.5308783011894362No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATC1500.503998387205161No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG1260.42335864525233524No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1080.3628788387877159No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCAT1050.3527988710436127No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG1040.34943888179557825No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC910.305759021571131No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT850.2855990860829245No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATC830.27887910758685575No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC820.2755191183388213No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC790.2654391505947181No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG770.25871917209864925No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC720.24191922585847728No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGTCAGGGGTATCTAATCCCAG720.24191922585847728No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA720.24191922585847728No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCAT680.22847926886633962No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATC660.2217592903702708No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT660.2217592903702708No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC660.2217592903702708No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGCGGGGTATCTAATCCCAGT640.215039311874202No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATC630.21167932262616762No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA630.21167932262616762No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCA630.21167932262616762No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGCACATGGGGTATCTAATCCCA590.19823936563402997No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC580.19487937638599556No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATC580.19487937638599556No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATC570.19151938713796116No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGAGTGGGGGTATCTAATCCCAG560.18815939788992675No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT560.18815939788992675No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG530.17807943014582353No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC520.17471944089778912No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC500.1679994624017203No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT490.1646394731536859No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT470.1579194946576171No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCA460.1545595054095827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAG460.1545595054095827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCAT460.1545595054095827No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCCGTGGGGGTATCTAATCCCA450.15119951616154828No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA450.15119951616154828No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA430.14447953766547947No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCA430.14447953766547947No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTC410.13775955916941066No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTGGGGTATCTAATCCCAG410.13775955916941066No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCA400.13439956992137625No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCC390.13103958067334184No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCA380.12767959142530744No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCC350.1175996236812042No Hit
TTCTCAGCTAGAACAGGCTCCTCTATTCGTGGGGGTATCTAATCCCAGTC350.1175996236812042No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT350.1175996236812042No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC340.11423963443316981No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC320.107519655937101No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCC320.107519655937101No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAG300.10079967744103219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAG29500.0294.05
TCTCAGC29750.0293.51262
TTCTCAG29750.0293.51261
CTCAGCT29700.0293.51013
TCAGCTA29700.0292.516854
GCTAGAA29400.0291.98987
AGCTAGA29700.0291.026956
CTAGAAC29500.0291.08
TAGAACA30650.0278.637829
ACGACCG150.0028358204196.66666295
GCTAGAC359.903488E-7168.571437
GGGGCAC452.7284841E-1159.00000435-39
GTTTGGG452.7284841E-1159.00000430-34
TTAGGGC7500.059.000004115-119
GCGGTTT900.059.00000485-89
GTTTACG1800.059.000004110-114
TTTAACT6500.059.000004100-104
CGGTTTT900.059.00000490-94
AAGGTTT1350.059.000004110-114
GGTTTGG452.7284841E-1159.00000430-34
Produced by FastQC (version 0.11.5)