FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-131-6-7-5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-131-6-7-5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28732
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1746860.79632465543645No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT15795.495614645691215No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT11153.8806905192816368No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT10203.5500487261589866No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT8552.975776138103856No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT4151.4443825699568427No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT3861.343449812056244No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT3771.3121258527077821No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT2420.8422664624808576No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT2280.7935403034943617No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT2200.7656967840735069No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2060.716970625087011No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT1720.598635667548378No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT1460.5081442294306No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCACCAAGTCCT1190.4141723513852151No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTACT990.3445635528330781No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCT780.27147431435333425No Hit
TGCTTATAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTTC780.27147431435333425No Hit
TGCTTATAGAACAGGCTCCTCTACAGGGGCTCGCTGGTCTGCCAAGCACG620.2157872755116247No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGCCTGAGGCGCCGCCAAGTCCT620.2157872755116247No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATGTGAAGCACCGCCAGGTCCT540.18794375609076988No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCCT540.18794375609076988No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC490.17054155645273564No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT470.16358067659752193No Hit
TGCTTATAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAAGTTTC450.15661979674230822No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT400.13921759710427398No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT390.13573715717666712No Hit
TGCTTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT380.13225671724906027No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT350.12181539746623973No Hit
TGCTTATAGAACAGGCTCCTCTAGAGTGATATAAAGCACTGCCAAGTCCT350.12181539746623973No Hit
TGCTTATGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT340.11833495753863288No Hit
TGCTTATAGAACAGGCTCCTCTAGAGTGATATGAAGCACCGCCAGGTCCT320.11137407768341918No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCAAGGTCCT300.10441319782820548No Hit
TGCTTATAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT290.10093275790059864No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAA108.422152E-4295.0295
CTTTAGA108.422152E-4295.03
TGCTTTA157.1671857E-6295.01
CTTATAA108.422152E-4295.03
TTAAGAA157.1671857E-6295.04
GAAAGGC108.422152E-4295.09
TTATAGA28050.0294.474154
CTTATAG28050.0294.474153
TGCTTAT28250.0293.955751
GAACAGG27550.0291.25239
GCTTATA28550.0290.866882
TATAGAA28300.0289.7885
ATAGAAC28350.0285.634926
TAGAACA28400.0284.612677
AGAACAG28800.0280.147588
TGCTTAA403.274181E-11221.251
CTTAAGA202.2590948E-5221.253
GCTTAAA202.2590948E-5221.252
GAAAAGG359.897431E-7168.571439
CCAACGA200.0067037437147.5295
Produced by FastQC (version 0.11.5)