FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-130-5-1-3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-130-5-1-3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29025
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT872630.06373815676141No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT294310.13953488372093No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTA20236.969853574504738No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCT15625.381567614125753No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT8592.959517657192076No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT7222.487510766580534No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGA6272.160206718346253No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTA6242.149870801033592No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATC4531.5607235142118863No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT4491.5469422911283377No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCG4171.4366925064599483No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT3341.1507321274763136No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTA3321.1438415159345392No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT3061.0542635658914727No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC3051.0508182601205858No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGA2820.9715762273901809No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGAT2800.9646856158484065No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAAC2520.8682170542635658No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTA2330.8027562446167097No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCG2310.7958656330749355No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2030.6993970714900948No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1930.664944013781223No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1650.5684754521963824No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT1480.5099052540913006No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTAA1430.4926787252368648No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACC1390.47889750215331606No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1090.3755383290267011No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1040.35831180017226527No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1030.35486649440137813No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCG960.330749354005168No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGA860.2962962962962963No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA810.27906976744186046No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGTA810.27906976744186046No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTAAC790.2721791559000862No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG760.26184323858742464No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT680.23428079242032732No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA650.2239448751076658No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT630.21705426356589144No Hit
TGCTTATAGAACAGGCTCCTCTAGCTTGGGGTATCTAATCCCAGTAACCG600.20671834625322996No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAA590.20327304048234282No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT580.19982773471145565No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT570.19638242894056845No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGAT560.1929371231696813No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCGA540.18604651162790697No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGA520.17915590008613264No Hit
TGCTTATAGAACAGGCTCCTCTAGGTATGCAGCGTGTGGAGCAACGGTTG510.17571059431524547No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT510.17571059431524547No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAA500.17226528854435832No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTA500.17226528854435832No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTA490.16881998277347116No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACC490.16881998277347116No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAA480.165374677002584No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA460.15848406546080965No Hit
TGCTTATAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGTAA460.15848406546080965No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCGA450.15503875968992248No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCG440.1515934539190353No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAACC440.1515934539190353No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT420.14470284237726097No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAA420.14470284237726097No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTA400.13781223083548663No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG390.1343669250645995No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT380.13092161929371232No Hit
TGCTTATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTAACCG380.13092161929371232No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGA370.12747631352282515No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTAACCGAT360.124031007751938No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT340.11714039621016366No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAAGTCCT330.1136950904392765No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT330.1136950904392765No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAAC320.11024978466838932No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT320.11024978466838932No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACC310.10680447889750216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT29000.0294.49141
CTTATAG28700.0293.97213
GCTTATA29100.0292.97252
GAACAGG28650.0292.940649
TTATAGA28900.0291.93774
TATAGAA29450.0286.485575
ATAGAAC29550.0285.516086
TAGAACA29550.0284.517767
AGAACAG29700.0283.08088
AGAACGG150.0028354523196.666668
GAACGGC150.0028354523196.666669
CAAGCGC150.0028354523196.66666295
CTTATAT352.1019715E-4126.4285663
CTTATAA352.1019715E-4126.4285663
TTATATA403.576233E-4110.6254
CGACAAG508.684181E-488.5295
GAAACCA508.684181E-488.5295
TTATAAA700.003300415863.2142834
ACTATTG452.7284841E-1159.000004100-104
GGGCTCG452.7284841E-1159.00000430-34
Produced by FastQC (version 0.11.5)