FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-13-4-5-7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-13-4-5-7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11657
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT190916.376426181693404No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC165014.154585227760144No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT9408.063824311572446No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT6215.327271167538818No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA5344.580938491893283No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC3002.5735609505018444No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2872.4620399759800975No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC2832.4277258299734066No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2622.2475765634382774No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCC2382.0416916873981297No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG2181.8701209573646733No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA1621.389722913270996No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1621.389722913270996No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1341.1495238912241572No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT1201.0294243802007377No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1160.9951102341940465No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCC930.7978038946555718No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT810.694861456635498No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA770.6605473106288068No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC760.6519687741271339No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATC750.6433902376254611No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAAT740.6348117011237883No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC730.6262331646221155No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCAT720.6176546281204427No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCA680.5833404821137514No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT600.5147121901003688No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA550.47181950759200475No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG540.463240971090332No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATC540.463240971090332No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT540.463240971090332No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAG480.4117697520802951No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC450.3860341425752767No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCATC440.37745560607360384No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA440.37745560607360384No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGCTGGGGGTATCTAATCCCAGTCA440.37745560607360384No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT420.3602985330702582No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC420.3602985330702582No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCAT380.32598438706356697No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGTCGGGGTATCTAATCCCAGTCA360.30882731406022135No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT360.30882731406022135No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCAT360.30882731406022135No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCATC340.2916702410568757No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG340.2916702410568757No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCA330.2830917045552029No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA330.2830917045552029No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC330.2830917045552029No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC330.2830917045552029No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTCAT330.2830917045552029No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTC320.27451316805353004No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTC310.26593463155185726No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC300.2573560950501844No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTAA290.24877755854851163No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCA280.2401990220468388No Hit
GAGACTTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTCAT270.231620485545166No Hit
GAGACTTAGAACAGGCTCCTCTAGCTTGGGGTATCTAATCCCAGTCATCC270.231620485545166No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA260.22304194904349317No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCA250.21446341254182036No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTTAAGAGACAGGGGGTATCTAATC240.20588487604014755No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCA240.20588487604014755No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAA230.19730633953847473No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCATC230.19730633953847473No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCATC230.19730633953847473No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC210.1801492665351291No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT200.1715707300334563No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT200.1715707300334563No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT180.15441365703011067No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCATC180.15441365703011067No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGCTGGGGTATCTAATCCCAGTC170.14583512052843786No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGGTATCTAATCCCAGTCATCC160.13725658402676502No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTTGGCGGGGGTATCTAATCCCAGT160.13725658402676502No Hit
GAGACTTAGAACAGGCTCCTCTAGCGCTCTTCCGATCTCGGGGTATCTAA160.13725658402676502No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT150.1286780475250922No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT150.1286780475250922No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGTATCTAATCCCAGTCATCC130.11152097452174659No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTAGTGGGCTCGGGGTATCTAATC130.11152097452174659No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTCA120.10294243802007377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC11350.0295.06
CTTAGAA11400.0293.706125
GAGACTT11550.0292.44591
GAACAGG11500.0292.434759
AGACTTA11500.0292.434752
GACTTAG11450.0292.423553
ACTTAGA11600.0288.642244
AGAACAG11950.0281.42268
TAGAACA12150.0278.004127
CAGTAGG200.0066529224147.49998295
CAGCGCA200.0066529224147.49998295
GAGACAA700.003250862763.214283295
TGTTGCT452.5465852E-1159.00000460-64
GTTGAGG1550.059.000004105-109
GTTCTGG1900.059.00000475-79
AGAGGGA2250.059.00000420-24
CCGCCAG1900.059.00000435-39
TTCTGGC1900.059.00000475-79
CGCCAGG1900.059.00000440-44
CAATTGT452.5465852E-1159.00000490-94
Produced by FastQC (version 0.11.5)