FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-129-5-1-3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-129-5-1-3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28957
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC877530.30355354491142No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC26169.034085022619747No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG25168.688745381082294No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGAT16035.535794453845357No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC8322.8732258175916012No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACC7682.652208447007632No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC7612.6280346721000103No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG4271.4746002693649203No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC3971.3709983769036849No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGA3921.3537313948268122No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3581.2363159167040785No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAAC3561.2294091238733293No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACC3211.1085402493352212No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAAC3191.101633456504472No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2921.00839175328936No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA2911.0049383568739856No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2850.9842179783817384No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT2390.8253617432745105No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2230.7701074006285181No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT2120.7321200400593985No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1820.6285181475981628No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCG1690.583623994198294No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1560.5387298407984252No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACC1490.5145560658908036No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1380.47656870532168394No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT1340.4627551196601858No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC1320.4558483268294367No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA1300.44894153399868775No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC1100.3798736056911973No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTA1030.35569983078357564No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC1020.35224643436820113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTA990.34188624512207755No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT950.32807265946057945No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC890.30735228096833234No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA850.2935386953068343No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACC810.2797251096453362No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC800.27627171322996164No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAAC800.27627171322996164No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCG790.2728183168145871No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACC780.2693649203992126No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTA760.26245812756846354No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA760.26245812756846354No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCA700.24173774907621645No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC690.23828435266084197No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG670.2313775598300929No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG630.2175639741685948No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT580.20029699209172222No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG570.19684359567634768No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAAC530.18303001001484961No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAA520.17957661359947508No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAAC500.17266982076872606No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC490.16921642435335152No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCCTGGGGTATCT480.165763027937977No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG430.1484960458611044No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAA420.14504264944572987No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC420.14504264944572987No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT420.14504264944572987No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC390.1346824601996063No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCA390.1346824601996063No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACC380.13122906378423177No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACC360.12432227095348276No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC360.12432227095348276No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATC350.12086887453810823No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAGGTC340.11741547812273372No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC330.11396208170735918No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC300.10360189246123562No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGCGGGGTATCTAATCCCAGTA290.10014849604586111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCT28650.0295.03
AATCTAG28700.0293.45825
CTCAATC28850.0292.954962
ATCTAGA28700.0292.43036
TCTCAAT28950.0291.943021
TCTAGAA28750.0291.921757
CAATCTA29000.0291.439644
CTAGAAC29000.0289.405158
TAGAACA30050.0278.8029
CTCACTC150.0028354272196.666672
TCACTCT200.0067040455147.499983
AACGGAA200.0067040455147.49998295
TCTCACT200.0067040455147.499981
GGGTCTG3100.059.00000425-29
GTTGAGG9350.059.000004105-109
TTGTAGT4550.059.00000470-74
GGCGAGC9800.059.00000480-84
GCAGTTT8500.059.00000485-89
TGCTTGT1800.059.00000465-69
CTTGTAG4550.059.00000470-74
Produced by FastQC (version 0.11.5)