FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-128-5-1-3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-128-5-1-3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28790
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC833528.95102466134074No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC26919.346995484543244No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT19476.76276484890587No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATC18166.307745745050365No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC8152.8308440430705106No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC6372.212573810350816No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT6362.2091003820771102No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCG5922.05626953803404No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT3781.312955887460924No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGAT3531.2261201806182702No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACC3471.2052796109760333No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC3261.1323376172282043No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC3211.1149704758596735No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGA3121.0837096213963182No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCG3101.076762764848906No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACC3101.076762764848906No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2820.9795067731851338No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2700.93782563390066No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2620.9100382077110107No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2470.8579367836054185No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2430.8440430705105939No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACC2360.8197290725946509No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2250.7815213615838833No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAA1990.6912122264675234No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTA1660.5765890934352206No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1570.5453282389718652No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1470.5105939562348037No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTA1210.4202848211184439No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTA1080.37513025356026397No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT1080.37513025356026397No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCG1050.3647099687391455No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1030.35776311219173323No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCG1030.35776311219173323No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG860.29871483153872874No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC860.29871483153872874No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT850.2952414032650226No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC850.2952414032650226No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA810.28134769017019795No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTAACCGA650.22577283779089963No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTA640.22229940951719349No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCAG640.22229940951719349No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCG640.22229940951719349No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAAC620.21535255296978115No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA600.20840569642236886No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACC590.20493226814866272No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT580.20145883987495658No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC570.19798541160125044No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC560.1945119833275443No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC550.19103855505383813No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC540.18756512678013199No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTA510.17714484195901356No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC510.17714484195901356No Hit
CCACGTCTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTAACC490.17019798541160125No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTA490.17019798541160125No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT480.1667245571378951No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGGTATCTAATCCCAGTAA480.1667245571378951No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAAC460.15977770059048282No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT450.15630427231677665No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAAC430.14935741576936437No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACC420.14588398749565823No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCA410.1424105592219521No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGGGTATCTAATCCCAGTAACC410.1424105592219521No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGA380.13199027440083363No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG380.13199027440083363No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC370.1285168461271275No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCTCGGGGGTATCTAATCCCAGT350.12156998957971517No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCG330.11462313303230288No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAAC300.10420284821118443No Hit
CCACGTCTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGAGGGGTATCT290.10072941993747829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA108.4222294E-4295.04
CCACGTC28500.0295.01
CACGTCT28500.0294.482482
ACGTCTA28350.0294.47973
CGTCTAG28300.0293.95764
TCTAGAA28050.0293.94836
GTCTAGA28400.0292.403175
CTAGAAC28550.0289.833627
TAGAACA29450.0280.475378
AGAACAG29700.0277.617839
ACGTCTG200.0067038047147.53
AGACAGG508.683411E-488.59
TGAGACA550.001267922980.45455295
CTTAAGC303.321893E-759.00000455-59
CTAGAAT150.00437272559.000004280-284
CAGCGGG150.00437272559.00000430-34
TCGTTTT150.00437272559.000004195-199
GCCTTTT150.00437272559.00000445-49
CCCTCGA303.321893E-759.00000425-29
CGAGTTT150.00437272559.000004110-114
Produced by FastQC (version 0.11.5)