FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-127-4-7-5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-127-4-7-5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28484
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT584420.516781350933858No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG303010.637550905771661No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC18766.586153630108131No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT17766.235079342788934No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTCT12794.4902401348125265No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCG6042.1204886954079485No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTCT5842.050273837944109No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT5762.022187894958573No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCTG5712.0046341805926136No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT5031.7659036652155597No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTTC4701.6500491504002246No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTGT4591.611430978795113No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTTC3561.2498244628563404No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC3361.1796096053925011No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT3331.1690773767729252No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTTC2780.975986518747367No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGCT2500.8776857182979918No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG2480.870664232551608No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT2480.870664232551608No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC2300.8074708608341524No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCG2280.8004493750877686No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCG2160.758320460609465No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT2110.7407667462435051No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC1900.6670411459064738No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1870.6565089172868979No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTCT1690.5933155455694424No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCGC1680.5898048026962506No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTCG1600.5617188597107148No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTCT1540.5406544024715629No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCGCT1460.5125684594860273No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1280.4493750877685719No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCTT1280.4493750877685719No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCTT1240.435332116275804No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCGC1180.4142676590366522No Hit
CTAGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTTCTG1130.3967139446706923No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTCT1120.39320320179750035No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTTC1120.39320320179750035No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTCT1080.3791602303047325No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC1050.3686280016851566No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCGC1030.36160651593877263No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTCGC1030.36160651593877263No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTCG1020.35809577306558066No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGGTATCTAATCC990.3475635444460048No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG990.3475635444460048No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT980.3440528015728128No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCGC910.319477601460469No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCAGT890.3124561157140851No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC880.30894537284089313No Hit
CTAGGTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCTTC850.29841314422131726No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCTT850.29841314422131726No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCGCTTC840.2949024013481253No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT810.28437017272854936No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTC770.27032720123578147No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTCT750.2633057154893976No Hit
CTAGGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCGCT740.2597949726162056No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTCT690.24224125825024573No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT680.23873051537705378No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA670.2352197725038618No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGCTT620.217666058137902No Hit
CTAGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCGC580.20362308664513412No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG580.20362308664513412No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCGCT570.20011234377194215No Hit
CTAGGTAGAACAGGCTCCTCTAGGTACGGGGTATCTAATCCCAGTCGCTT560.19660160089875017No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG550.19309085802555823No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTCG550.19309085802555823No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCGC510.17904788653279033No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT480.16851565791321443No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGTGGGGTATCTAATCCCAGTCG470.16500491504002246No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCTT470.16500491504002246No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT440.15447268642044656No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG430.15096194354725462No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC430.15096194354725462No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGTCGGGGTATCTAATCCCAGTCG400.1404297149276787No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT390.13691897205448672No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTCGCT380.13340822918129477No Hit
CTAGGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCGCT370.1298974863081028No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT360.12638674343491083No Hit
CTAGGTAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCTAAT330.11585451481533492No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT310.108833029068951No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCGCTT300.10532228619575904No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT290.10181154332256706No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTCGCTTC290.10181154332256706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCT108.421766E-4295.09
AGGTAGA28150.0293.428073
TAGGTAG28150.0293.428072
GGTAGAA28000.0293.419654
GAACAGG27750.0291.810828
CTAGGTA28500.0290.859651
AACAGGC27900.0290.241949
GTAGAAC28500.0286.71935
AGAACAG28950.0281.24357
TAGAACA29500.0275.56
GACAGGC202.2588882E-5221.258
GGTAGAC202.2588882E-5221.254
GAACGGC150.0028351697196.666678
GGTAAAC200.0067034382147.54
TAGGTAA200.0067034382147.52
GATAGTG300.001359914598.333336295
AGGACGA508.682469E-488.5295
GTAGAAA600.001790737773.755
CTAGGGA402.415989E-673.751
AGACAGG700.003299769563.2142837
Produced by FastQC (version 0.11.5)