FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-126-5-7-5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-126-5-7-5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27555
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT878731.888949373979315No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT309511.232081291961531No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC17836.470694973688985No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT13024.72509526401742No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT8172.9649791326438035No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT7712.798040283070223No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTC5662.054073670840138No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT4201.5242242787152966No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT3861.400834694247868No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTG3861.400834694247868No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC3531.2810742152059518No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT3511.2738160043549265No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC3161.1467973144619852No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTC3091.1213935764833969No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2670.9689711486118672No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGC2420.8782435129740519No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCT2190.7947740881872618No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT2150.7802576664852113No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2100.7621121393576483No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC1550.5625113409544547No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1390.504445654146253No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1280.4645254944656142No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTT1180.4282344402104881No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT1170.4246053347849755No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCG1170.4246053347849755No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCT1160.42097622935946294No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTT1130.4100889130829251No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCT1060.38468517510433675No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCG1050.38105606967882416No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTC1030.373797858827799No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1000.36291054255126115No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT980.3556523317002359No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTCG730.26492469606242064No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTC720.261295590636908No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC710.2576664852113954No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTC710.2576664852113954No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT670.24315006350934493No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT670.24315006350934493No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCG670.24315006350934493No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCG580.21048811467973147No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA580.21048811467973147No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTCG580.21048811467973147No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT550.19960079840319359No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT550.19960079840319359No Hit
TGCTTATAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGTCG530.19234258755216838No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCG510.18508437670114317No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT480.17419706042460534No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA470.17056795499909272No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTC470.17056795499909272No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT460.1669388495735801No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTC460.1669388495735801No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCGCTT450.16330974414806748No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCT450.16330974414806748No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTC450.16330974414806748No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCGC430.1560515332970423No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGCT390.14153511159499182No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT380.1379060061694792No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT370.13427690074396662No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACTGGGGTATCTAATCCCAGTCGC360.130647795318454No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTC360.130647795318454No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG360.130647795318454No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTC350.1270186898929414No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT330.11976047904191617No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCAG330.11976047904191617No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTC320.11613137361640355No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT320.11613137361640355No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGCGGCACGCAGGGGTATCTAATCC320.11613137361640355No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA300.10887316276537834No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT290.10524405733986573No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGTTGGGGTATCTAATCCCAGTCG290.10524405733986573No Hit
TGCTTATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTC290.10524405733986573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT27500.0294.463621
GCTTATA27550.0293.929232
CTTATAG27450.0293.387973
GAACAGG26950.0292.263469
TTATAGA27650.0291.26584
TATAGAA27750.0288.621645
TAGAACA27700.0285.415167
ATAGAAC27850.0284.937166
AGAACAG27800.0283.85798
GAAAAGG255.498155E-5177.09
TAACAGG200.006702292147.59
ATGAGAG455.7099684E-498.333336295
AGCCTAG257.797844E-659.000004120-124
CGTCAGG150.004372337459.00000425-29
ACTGCCA1300.059.00000435-39
GTGTTGA150.004372337459.000004195-199
CGGAGGG150.004372337459.00000435-39
GCCTTAT150.004372337459.000004210-214
ATCTTGG150.004372337459.000004200-204
TAGGACT600.059.00000420-24
Produced by FastQC (version 0.11.5)