FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-125-5-7-5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-125-5-7-5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27408
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC918533.51211325160537No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC291510.635580852305896No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT19106.968768242848804No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG10873.9659953298307062No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC8443.079392877991827No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTT6762.4664331582019847No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC6232.273058960887332No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5021.8315820198482196No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCT4931.7987448920023352No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC4371.594424985405721No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC3321.2113251605370694No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT3111.1347051955633392No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC3031.1055166374781087No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCT2901.058085230589609No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCT2420.8829538820782252No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTC2180.7953882078225335No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2170.7917396380618797No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTT1710.6239054290718038No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCG1700.62025685931115No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCT1410.5144483362521891No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA1320.4816112084063047No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1240.45242265032107415No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTT1220.4451255107997665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGC1170.4268826619964973No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTC1060.3867483946293053No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1060.3867483946293053No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCT1020.37215411558669004No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGC1010.3685055458260362No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG940.3429655575014594No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC860.31377699941622883No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG860.31377699941622883No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCG830.30283129013426735No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA820.29918272037361354No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT820.29918272037361354No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTT730.26634559252772916No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC710.2590484530064215No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG660.24080560420315236No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT620.22621132516053707No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT610.22256275539988324No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC600.21891418563922943No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC540.19702276707530647No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC540.19702276707530647No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCG530.19337419731465266No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT530.19337419731465266No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGC530.19337419731465266No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG520.18972562755399883No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATC480.17513134851138354No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC480.17513134851138354No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT440.16053706946876825No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCGCT430.15688849970811442No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC400.14594279042615294No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA390.14229422066549913No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA390.14229422066549913No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG390.14229422066549913No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTC380.1386456509048453No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGTCAGGGGTATCTAATCCCAGT370.13499708114419148No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAA360.13134851138353765No Hit
GCGAAGTTAGAACAGGCTCCTCTATGTCTCGGGGGGTATCTAATCCCAGT350.12769994162288384No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC350.12769994162288384No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCG340.12405137186223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC340.12405137186223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTT340.12405137186223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGTGGGGTATCTAATCC340.12405137186223No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT330.12040280210157618No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTC320.11675423234092236No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC320.11675423234092236No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC300.10945709281961472No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTT300.10945709281961472No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGAAGCGTCAGGGGTATCTAATCCCA280.10215995329830707No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG280.10215995329830707No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGCTGGGGTATCTAATCCCAGT280.10215995329830707No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCG280.10215995329830707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTT27150.0294.456732
GAAGTTA27150.0293.913453
GCGAAGT27350.0293.38211
AAGTTAG27100.0293.367164
AGTTAGA27100.0293.367165
GTTAGAA27000.0293.36116
TTAGAAC27250.0290.128457
AGAACAG27950.0281.80689
TAGAACA28300.0278.321568
TCGACAA150.0028346196.66667295
GCGAATT150.0028346196.666671
CGAATTA150.0028346196.666672
AGAACGG455.709632E-498.3333369
TAGAACG508.6790096E-488.58
ACAGCGA700.003298464263.214283295
GATGGGG150.00437228759.00000425-29
CTCGAAA150.00437228759.000004255-259
AAACTTA150.00437228759.000004235-239
GCCTTTT150.00437228759.00000445-49
CAGTCTT150.00437228759.00000485-89
Produced by FastQC (version 0.11.5)