FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-124-5-1-3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-124-5-1-3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27297
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC843330.893504780745136No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC26009.524856211305272No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT24989.151188775323297No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATC13284.865003480235924No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC8112.9710224566802212No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC6842.50576986482031No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCG5592.0478440854306337No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACC3891.4250650254606734No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC3851.4104114005202038No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT3851.4104114005202038No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGA3361.2309044949994505No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCG3021.1063486830054585No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC2901.0623878081840497No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGAT2811.0294171520679927No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2350.8609004652525919No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2020.7400080594937173No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1820.6667399347913691No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1800.6594131223211342No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1750.6410960911455471No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTA1700.6227790599699601No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1680.6154522474997253No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACC1660.6081254350294905No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1640.6007986225592555No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1450.5311939040920248No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1240.45426237315455914No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTA1210.4432721544492068No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC1140.417628310803385No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAAC1120.41030149833315016No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1080.3956478733926805No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG890.32604315492544966No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACC880.32237974869033226No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTAA860.31505293622009745No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACC780.28574568633915814No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTAA760.27841887386892333No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCG750.2747554676338059No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTA720.26376524892845365No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT720.26376524892845365No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA690.25277503022310144No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA620.22713118657727957No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAAC620.22713118657727957No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTA620.22713118657727957No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT600.21980437410704473No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAA600.21980437410704473No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC550.20148734293145767No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG540.19782393669634024No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCG540.19782393669634024No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCG540.19782393669634024No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT530.19416053046122284No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC530.19416053046122284No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA520.19049712422610543No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGT510.18683371799098802No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTAAC480.17584349928563578No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT470.17218009305051837No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAAC450.16485328058028356No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC440.16118987434516613No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA430.15752646811004872No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTA420.15386306187493132No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT410.15019965563981388No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACC400.14653624940469648No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCG400.14653624940469648No Hit
GCGAAGTTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTAACCG390.14287284316957907No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAAC360.13188262446422683No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC360.13188262446422683No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATC330.1208924057588746No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA320.11722899952375718No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTA310.11356559328863979No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC290.10623878081840496No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCGGGGTATCTAATCCCAGT280.10257537458328754No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT280.10257537458328754No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTA280.10257537458328754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGG157.1632057E-6295.01
TTAAAAC108.4198156E-4295.07
CGAAGTT26800.0294.449622
GTTAGAA26500.0294.44346
GAAGTTA26800.0293.899263
AGTTAGA26700.0293.89515
GCGAAGT27000.0293.36111
AAGTTAG26800.0293.348884
TTAGAAC26900.0288.96847
AGAACAG28100.0276.62819
TAGAACA28350.0274.7098
GTTAGAC202.2578426E-5221.256
CGAAATT150.0028345147196.666672
AAGGTAG150.0028345147196.666674
GAAATTA200.006701893147.53
GCGAAAT200.006701893147.51
AACCATG200.006701893147.5295
CGAAGGT200.006701893147.52
TTAGACA352.100587E-4126.4285667
AGACAGG352.100587E-4126.4285669
Produced by FastQC (version 0.11.5)