FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-123-6-4-8-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-123-6-4-8-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26781
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1200144.811620178484745No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC24589.178148687502334No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCA14835.537507934729845No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC11104.14472947238714No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC10163.793734363914716No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC5982.232926328367126No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT5271.9678130017549755No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC5151.9230051155670065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT5141.9192711250513423No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC4841.8072514095814198No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATCTAAAGTACCGCCAAGTCC4771.7811134759717713No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC4741.7699115044247788No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATAAAGCACCGCCAAGTCC4241.5832119786415744No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCC4211.572010007094582No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGT3941.4711922631716514No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCC3701.3815764907957133No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC2570.9596355625256712No Hit
CGATTCCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTCAAT2550.9521675814943431No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATC1710.6385123781785594No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1420.5302266532243008No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTCAGTGTAGATGGCTACTGGCAGG1350.5040887196146522No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAA990.3696650610507449No Hit
CGATTCCTAGAACAGGCTCCTCTAGCATCACGATCTCGTATGCCGTCTTC750.2800492886748068Illumina PCR Primer Index 1 (96% over 26bp)
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACCGCCAAGTCC350.13068966804824317No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGATATGAAGCACCGCCAGGTCCT280.10455173443859453No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAG280.10455173443859453No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT270.10081774392293043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA26300.0295.05
CCTAAAC108.418951E-4295.06
TTCCTAG26350.0295.04
TTCCTAA108.418951E-4295.04
ATTCCTA26450.0295.03
CCCACGA157.161734E-6294.99997295
GATTCCT26550.0294.444462
CCTAGAA26350.0293.880466
CGATTCC26650.0293.33961
CTAGAAC26450.0292.769387
AGAACAG26900.0286.226759
TAGAACA27250.0283.633038
TCCTAAA150.0028342248196.666665
CGCAGAA200.0067012087147.5295
TGAGAGA200.0067012087147.5295
ACATGCA403.5731544E-4110.625295
AGTACAG508.676732E-488.5295
AGCAAGA806.0138686E-573.75295
CTTTTGA501.8189894E-1259.00000445-49
TGCATTA257.796609E-659.000004220-224
Produced by FastQC (version 0.11.5)