FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-122-6-7-5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-122-6-7-5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26534
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC1425453.71975578503053No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC26169.859048767618903No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC10784.062711992161001No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC10583.9873370015828744No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC9003.391874576015678No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC6102.2989372126328482No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC3091.1645436044320494No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTT2841.0703248662093916No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC2661.0024873746890781No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2090.7876686515414186No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGAGTCTGAGGCACCGCCAAGTC1210.4560186929976634No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC1070.40325619959297504No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC1050.39571870053516245No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1000.3768749528906309No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC990.3731062033617246No Hit
TCTCAATCTAGAACAGGCTCCTCTGCTTGTGCGGGTCCCCGTCTATTCCT960.36179995477500565No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT690.2600437174945353No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCT660.2487374689078164No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACCGTCTGGATCTTGCCATTGA560.21104997361875327No Hit
TCTCAATCTAGAACAGGCTCCTCTACCGCCCAGGCTATCGCTTACGGCTC520.1959749755031281No Hit
TCTCAATCTAGAACAGGCTCCTCTTGTCTGCCTTCCAGGAGGGAACAAAG500.18843747644531544No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC470.17713122785859653No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC460.1733624783296902No Hit
TCTCAATCTAGAACAGGCTCCTCGCAGTTGTCCTCAGAGCGATTTACTGC460.1733624783296902No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACGAACCAGTTGGTGATGCCCCA440.1658249792718776No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTC430.1620562297429713No Hit
TCTCAATCTAGAACAGGCTCCTCTCGGGCGGTTTGGCCAAAAACGCTGGT380.14321248209843973No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTT360.13567498304062714No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTC360.13567498304062714No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC360.13567498304062714No Hit
TCTCAATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC350.13190623351172082No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGTGCCCAACTTAATGCTGGCAAC330.1243687344539082No Hit
TCTCAATCTAGAACAGGCTCCTCTACTGTCGATGGAAGCGCGTCCATCAG320.12059998492500187No Hit
TCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC310.11683123539609558No Hit
TCTCAATCTAGAACAGGCTCCTCGCGGCATGTGCGTCTGGGCCGCAGCCT290.10929373633828297No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAAAGTTAACTTGACTTTAAGAAA290.10929373633828297No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC280.10552498680937664No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGTGGGTGGTGCCTTGTGTGTTCC280.10552498680937664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACA108.418506E-4295.08
TCTGAAC108.418506E-4295.07
TCCAACA108.418506E-4295.0295
CTCAATC25950.0294.43162
TCAATCT25750.0294.42723
CTAGAAC25600.0293.847668
TCTAGAA25600.0293.271487
AATCTAG25650.0292.69985
ATCTAGA25650.0292.69986
CAATCTA25800.0291.569764
TCTCAAT26500.0289.433961
TAGAACA26350.0285.483869
TGACCAA150.0028340758196.66666295
ACGCAGG150.0028340758196.66666295
CTGGAAC150.0028340758196.666668
TCTCAAA304.5853994E-7196.666661
CTCAATT150.0028340758196.666662
CTCAAGC255.4957945E-5177.02
CAATCTG359.883188E-7168.571434
TCTCAAG359.883188E-7168.571431
Produced by FastQC (version 0.11.5)