FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-121-5-5-7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-121-5-5-7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26515
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT623723.52253441448237No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC275710.397887987931359No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT18827.097869130680746No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT16896.369979257024326No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA8903.356590609089195No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC7342.7682443899679425No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC6022.2704129737884218No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT5522.081840467659815No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC5512.078069017537243No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCC4561.7197812558928907No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3661.3803507448613992No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA3291.2408070903262303No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC3061.1540637375070715No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2821.0635489345653404No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC2600.9805770318687534No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT2350.8862907788044503No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2280.8598906279464453No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2270.8561191778238733No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA2220.8372619272110126No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2050.7731472751272864No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1810.6826324721855553No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG1720.6486894210824062No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA1670.6298321704695455No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCAT1650.6222892702244013No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCA1630.614746369979257No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCATC1450.5468602677729587No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCC1320.49783141617952104No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1300.4902885159343767No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCA1240.467659815198944No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATC1230.4638883650763719No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCCA1070.4035451631152178No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGTC970.36583066188949653No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCAT970.36583066188949653No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTC960.3620592117669244No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTACAGGGGTATCTAATCCCAGTCAT940.3545163115217801No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC900.33943051103149163No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT820.3092589100509146No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA810.3054874599283425No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT810.3054874599283425No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT780.2941731095606261No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT770.2904016594380539No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCA750.2828587591929097No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTCAT710.2677729587026212No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCC660.24891570808976052No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCA660.24891570808976052No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTCA650.24514425796718836No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTCA650.24514425796718836No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCATC600.22628700735432775No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG590.22251555723175562No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTCAT570.21497265698661133No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTCATC570.21497265698661133No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA560.21120120686403923No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTCA550.2074297567414671No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCA520.19611540637375072No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC520.19611540637375072No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTC520.19611540637375072No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA500.18857250612860646No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAGT490.1848010560060343No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCCA490.1848010560060343No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA480.1810296058834622No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA450.16971525551574582No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG430.16217235527060156No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGTGGGGTATCTAATCCCAGTCAT420.1584009051480294No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTCAT410.1546294550254573No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT400.15085800490288515No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTCAT400.15085800490288515No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGCTCGGGGTATCTAATCCCAGTCA390.14708655478031304No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCATC390.14708655478031304No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGTGGGGGTATCTAATCCCAGTC350.1320007542900245No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTCA350.1320007542900245No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGCGGGGTATCTAATCCCA330.12445785404488026No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT330.12445785404488026No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCA300.11314350367716387No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT300.11314350367716387No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGGTATCTAATCCCAGTCAT300.11314350367716387No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT290.10937205355459173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAAGA108.418506E-4295.04
AGTGGAA108.418506E-4295.02
GAGTGGT26300.0292.756651
AGTGGTA26200.0292.748082
GTGGTAG26200.0292.185123
TGGTAGA26200.0292.185124
GGTAGAA26150.0291.615665
GAACAGG26150.0289.923529
GTAGAAC26700.0284.503756
TAGAACA27500.0277.300027
AGAACAG27550.0276.796728
GACAGGC150.0028340758196.666669
GTGGAAG150.0028340758196.666663
TGAGGAG150.0028340758196.66666295
CGAAGCC150.0028340758196.66666295
TCACAGA806.012826E-573.75295
GAACAGC600.001789373573.749999
ATTTAAC7200.059.00000495-99
CAGGTCC7150.059.00000440-44
CTAGAGG8400.059.00000420-24
Produced by FastQC (version 0.11.5)