FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-120-5-8-2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-120-5-8-2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26945
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC716126.57635925032474No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC23988.899610317313044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG23488.714047133048803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC13414.97680460196697No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT8543.169419187233253No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC6882.5533494154759695No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG6842.53850436073483No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA5191.9261458526628317No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC5111.8964557431805529No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG4211.5624420115049176No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG4201.5587307478196326No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGT4001.4845054741139359No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3101.1504917424383003No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG2961.0985340508443124No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC2881.0688439413620336No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT2701.0020411950269066No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA2520.9352384486917796No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2320.8610131749860828No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTT2230.8276118018185192No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2060.7645203191686769No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG1870.694006309148265No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1610.5975134533308591No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTG1400.5195769159398775No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1280.47504175171645946No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC1020.3785488958990536No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT1010.3748376322137688No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT990.3674151048431991No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC960.35628131378734457No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG960.35628131378734457No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA940.3488587864167749No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTG920.3414362590462052No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC900.33401373167563553No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA880.3265912043050659No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGT860.3191686769344962No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC850.31545741324921134No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA790.2931898311375023No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC790.2931898311375023No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT700.25978845796993877No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT700.25978845796993877No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT690.2560771942846539No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA670.24865466691408428No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC610.2263870848023752No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTGT610.2263870848023752No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGT590.21896455743180554No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT540.20040823900538135No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGT540.20040823900538135No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG530.1966969753200965No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTA530.1966969753200965No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGT490.18185192057895713No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCT460.1707181295231026No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGT460.1707181295231026No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC460.1707181295231026No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC450.16700686583781776No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTTGGGGTATCTAATCCCAG450.16700686583781776No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC440.16329560215253294No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG420.15587307478196324No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTG420.15587307478196324No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGT420.15587307478196324No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGTCGGAGGGGTATCT410.15216181109667842No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTG410.15216181109667842No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC390.14473928372610873No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG390.14473928372610873No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTG370.13731675635553905No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC370.13731675635553905No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG370.13731675635553905No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTG360.1336054926702542No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC340.12618296529968456No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC340.12618296529968456No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGT340.12618296529968456No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT340.12618296529968456No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGT330.12247170161439971No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT330.12247170161439971No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAG330.12247170161439971No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC290.10762664687326035No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG290.10762664687326035No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTGTG280.10391538318797551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCTA26450.0295.04
CTCAGCT26650.0294.44653
TCTCAGC26850.0293.90132
GCTAGAA26400.0293.882577
TTCTCAG27000.0293.36111
CAGCTAG26500.0293.33025
CTAGAAC26500.0292.77368
AGCTAGA26600.0292.227426
TAGAACA27750.0279.585579
GTGTCGA150.002834313196.66666295
CAGCTAA200.006701417147.55
TCAGCTG301.1367371E-4147.499984
GCTGAAC403.5733756E-4110.6257
CAGAGGA508.6772686E-488.5295
AGCTGAA550.001267027580.454556
CAGCTGA600.001789669573.749995
CAAGATT452.7284841E-1159.000004110-114
GCTAGGC2850.059.000004120-124
GGCGCTT2850.059.00000465-69
TCGGTAA452.7284841E-1159.00000495-99
Produced by FastQC (version 0.11.5)