FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-12-5-2-6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-12-5-2-6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11585
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT317727.423392317652134No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT11299.745360379801467No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG9107.854984894259818No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT5995.170479067760034No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT3673.1678895123003885No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2752.373759171342253No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC2712.339231765213638No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG2342.0198532585239537No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC2191.8903754855416486No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT1661.4328873543375054No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1651.4242555028053518No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTT1641.4156236512731981No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG1381.1911955114372033No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTTG1221.0530858869227449No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1181.0185584807941304No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGTG1171.0099266292619768No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1070.9236081139404403No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1060.9149762624082866No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1030.8890807078118257No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC950.8200258955545966No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGCC720.6214933103150626No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC710.612861458782909No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA670.5783340526542944No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTTG650.561070349589987No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTT600.5179110919292188No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT550.4747518342684506No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT530.4574881312041433No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTGT470.40569702201122143No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGGC460.39706517047906775No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTTG440.37980146741476045No Hit
CTAGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTTGT440.37980146741476045No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTGT410.35390591281829953No Hit
CTAGGTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCCC400.3452740612861459No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG390.3366422097539922No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTGT390.3366422097539922No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTT370.3193785066896849No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTTG340.293482952093224No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT290.2503236944324558No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG280.2416918429003021No Hit
CTAGGTAGAACAGGCTCCTCTAGGTAAAGTCATGTATCTACGGTAGGGGT270.23305999136814848No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG270.23305999136814848No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGTGG260.22442813983599483No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT250.21579628830384118No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT240.20716443677168753No Hit
CTAGGTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTTG230.19853258523953388No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT220.18990073370738023No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGTCGGGGTATCTAATCCCAGTTG220.18990073370738023No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCCT210.18126888217522658No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG200.17263703064307295No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCCC200.17263703064307295No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGTCGGGGTATCTAATCCCAGTT200.17263703064307295No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT190.1640051791109193No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA190.1640051791109193No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT190.1640051791109193No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTTGT190.1640051791109193No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAGTT180.15537332757876565No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT180.15537332757876565No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT180.15537332757876565No Hit
CTAGGTAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGTTGT170.146741476046612No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA170.146741476046612No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT170.146741476046612No Hit
CTAGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG160.13810962451445835No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTGTG160.13810962451445835No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTTGT160.13810962451445835No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGCCGGGGTATCTAATCCCAGT160.13810962451445835No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG160.13810962451445835No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTTGT160.13810962451445835No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT150.1294777729823047No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAGT150.1294777729823047No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT140.12084592145015105No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTGTGG140.12084592145015105No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAGTT140.12084592145015105No Hit
CTAGGTAGAACAGGCTCCTCTAGCGCTCTTCCGATCTAGGGGTATCTAAT140.12084592145015105No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT120.10358221838584376No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT120.10358221838584376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA108.3570444E-4295.04
GTTAGAA108.3570444E-4295.05
AGGTTAG108.3570444E-4295.03
TAGGTTA108.3570444E-4295.02
CTAGGTA11400.0293.706121
TAGGTAG11350.0293.700442
GGTAGAA11250.0293.688874
AGGTAGA11400.0292.412263
AACAGGC11200.0291.04919
GTAGAAC11300.0289.778755
GAACAGG11300.0288.473458
TAGAACA11950.0274.016756
AGAACAG12100.0270.619847
TTAGAAC200.0066521815147.56
GGTAGAC200.0066521815147.54
CTAGGTT402.390978E-673.751
CTACAGG452.5465852E-1159.00000425-29
ACTGCCA452.5465852E-1159.00000435-39
GGCACCG1650.059.00000430-34
TACAGGG452.5465852E-1159.00000425-29
Produced by FastQC (version 0.11.5)