FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-119-4-8-2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-119-4-8-2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27438
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT450716.42612435308696No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT336212.253079670529923No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTGT17636.425395436985203No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTGC15555.6673226911582475No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT13985.095123551279248No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT8843.2218091697645597No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTGC5461.9899409577957576No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCTG4771.7384649026897003No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT4281.5598804577593117No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTTG3991.4541876230045923No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG3951.4396093009694584No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTTG3951.4396093009694584No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT3811.3885851738464903No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT3511.2792477585829871No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTTG3351.220934470442452No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG3261.188133245863401No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG3021.1006633136525985No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGCT2610.9512355127924775No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT2240.8163860339674903No Hit
AGGAATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTG2200.8018077119323566No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1850.6742473941249362No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTGC1700.6195786864931847No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTGT1690.6159341059844012No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGTG1610.5867774619141337No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1560.5685545593702165No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT1530.5576208178438662No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTTG1490.5430424958087324No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1370.49930752970333114No Hit
AGGAATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTTGC1350.49201836868576426No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGTT1260.4592171441067133No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA1210.4409942415627961No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTGTT1150.4191267585100955No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1150.4191267585100955No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTG1140.41548217800131204No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGTGT1070.38997011443982793No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG1060.3863255339310446No Hit
AGGAATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTGT1010.36810263138712734No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGTG1000.36445805087834393No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT970.3535243093519936No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGCT960.34987972884321017No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTTGC910.331656826299293No Hit
AGGAATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTGC900.3280122457905095No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT890.32436766528172606No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTG880.32072308477294265No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT870.3170785042641592No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA740.2696989576499745No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTGTT730.26605437714119107No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT690.2514760551060573No Hit
AGGAATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGTT680.24783147459727387No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTGTT670.2441868940884904No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT640.2332531525621401No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT620.22596399154457322No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT610.22231941103578978No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGTG590.21503025001822293No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTGTGTTGC590.21503025001822293No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTG570.20774108900065602No Hit
AGGAATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTGTTG570.20774108900065602No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTTGC560.20409650849187258No Hit
AGGAATAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTGT540.19680734747430573No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG530.1931627669655223No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTGTT520.18951818645673882No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG510.18587360594795538No Hit
AGGAATAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGTG510.18587360594795538No Hit
AGGAATAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGTG510.18587360594795538No Hit
AGGAATAGAACAGGCTCCTCTAGAACTACAGGGGTATCTAATCCCAGTGT510.18587360594795538No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTTGC510.18587360594795538No Hit
AGGAATAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCCC510.18587360594795538No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTGGAGGGGTATCTAAT490.1785844449303885No Hit
AGGAATAGAACAGGCTCCTCTAGCACCCTTTTCATAGGGCTGGGGGTATC490.1785844449303885No Hit
AGGAATAGAACAGGCTCCTCTAGGACTGGGGTATCTAATCCCAGTGTGTT470.17129528391282164No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTGTG470.17129528391282164No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGCAGTCAGGGGTATCTAATCCCAG460.16765070340403818No Hit
AGGAATAGAACAGGCTCCTCTAGCTGGGGTATCTAATCCCAGTGTGTTGC450.16400612289525476No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT450.16400612289525476No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTGTT450.16400612289525476No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTCGGGGTATCTAATCCCAGT440.16036154238647132No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGTT430.15671696187768785No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTGTGT390.14213863984255412No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTGTGT390.14213863984255412No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG380.13849405933377068No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGTTG380.13849405933377068No Hit
AGGAATAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTGTTG370.13484947882498724No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA340.12391573729863693No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT330.12027115678985348No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGTG320.11662657628107005No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTAATC310.11298199577228661No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGCTTGGGGTATCTAATCC300.10933741526350316No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT290.10569283475471973No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTGT290.10569283475471973No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGCGT290.10569283475471973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAG27300.0293.379152
GAACAGG27100.0291.190038
AGGAATA27600.0290.190221
AACAGGC27200.0290.119489
GAATAGA27800.0288.102543
ATAGAAC28050.0283.95725
TAGAACA28000.0282.357156
AATAGAA28500.0279.99124
AGAACAG28700.0275.98437
GAACCGG150.0028346196.666678
AGGAATG200.006702095147.499981
ACACGCA804.12183E-9110.62499295
TAGAACC455.709632E-498.3333366
GGGGCAC452.7284841E-1159.00000430-34
TATTTCT1250.059.00000495-99
GTTTGGG452.7284841E-1159.00000425-29
ATGGGGA150.00437228759.000004230-234
CTGTCGC150.00437228759.000004155-159
TGCCAGA150.00437228759.000004215-219
GCTGGGT150.00437228759.000004190-194
Produced by FastQC (version 0.11.5)