FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-118-5-2-6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-118-5-2-6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26387
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC869232.940463106832915No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC296411.232804032288627No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT14535.506499412589533No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT13445.093417213021564No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC9073.4372986697995227No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC6522.470913707507485No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG6512.46712396255732No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC4391.6636980331223707No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT3791.4363133361124798No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC3471.3150414977072042No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG3141.1899799143517642No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC2821.0687080759464889No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG2630.9967029218933566No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGC2580.9777541971425323No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2510.9512259824913784No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG2200.8337438890362678No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG1970.7465797551824762No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1890.7162617955811573No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1790.6783643460795088No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTT1750.6632053662788494No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTG1480.5608822526243984No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1240.4699283738204419No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1200.45476939401978245No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1180.44718990411945275No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1130.4282411793686285No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC1110.4206616894682988No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTG960.36381551521582595No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC870.3297078106643423No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC870.3297078106643423No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGT850.32212832076401254No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGG810.30696934096335315No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGC810.30696934096335315No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT750.28423087126236407No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT700.26528214651153975No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTTG660.25012316671088036No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT630.2387539318603858No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTTG600.22738469700989122No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC580.21980520710956153No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGT560.2122257172092318No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGG560.2122257172092318No Hit
CGATTCCTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTT560.2122257172092318No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA550.20843597225906696No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG540.2046462273089021No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC520.1970667374085724No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGG500.1894872475082427No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTG500.1894872475082427No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTT480.18190775760791297No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTT470.17811801265774813No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC430.16295903285708874No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG390.1478000530564293No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC380.14401030810626445No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA380.14401030810626445No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT370.1402205631560996No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC370.1402205631560996No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGG360.13643081820593475No Hit
CGATTCCTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGTT340.12885132830560503No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC340.12885132830560503No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTT340.12885132830560503No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTTG340.12885132830560503No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG310.11748209345511047No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC310.11748209345511047No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGT300.11369234850494561No Hit
CGATTCCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA290.10990260355478076No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTGT290.10990260355478076No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTT290.10990260355478076No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT270.10232311365445106No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC270.10232311365445106No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGCGGGGTATCTAATCCCAGTTG270.10232311365445106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCT25950.0295.000032
TTCCTAC108.418235E-4295.04
CGATTCC26000.0295.01
TTCCTAG25650.0294.424964
TCCTAGA25600.0293.847665
CCTAGAA25550.0293.84546
ATTCCTA26050.0293.301333
CTAGAAC25600.0290.96687
TAGAACA26150.0284.847058
AGAACAG26950.0276.391489
TTCCTAA251.8477294E-7236.04
TCCTACA150.0028339843196.666675
TCCTAAA304.5849083E-7196.666675
ACTCGAA200.006700642147.5295
CCTAAAC301.13647395E-4147.56
GCAGGCA200.006700642147.5295
CGATTCT200.006700642147.51
CGATTAC200.006700642147.51
CTAGAAA601.442762E-598.3333367
GCCCTCG150.00437191559.00000425-29
Produced by FastQC (version 0.11.5)