FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-117-5-7-5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-117-5-7-5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25829
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT843232.6454760153316No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT287711.138642611018621No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC18747.255410585001355No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT9403.6393201440241585No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT8383.2444151922257927No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT6942.6869023190986874No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTC4881.8893491811529675No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT3981.5409036354485268No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC3121.2079445584420612No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCT3051.1808432382206047No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2651.0259785512408532No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2641.0221069340663596No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC2350.9098300360060396No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT2310.8943435673080646No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2190.8478841612141392No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2180.8440125440396453No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTT2030.7859382864222386No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGC1750.6775330055364126No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCT1740.6736613883619188No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1740.6736613883619188No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1690.6543033024894498No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1550.6001006620465368No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTC1500.5807425761740679No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG1340.5187967013821673No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTT1310.507181849858686No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTG1270.49169538116071076No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1080.41813465484532886No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT990.3832901002748848No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCT940.3639320144024159No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTCG870.3368306941809594No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC850.3290874598319718No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCG800.3097293739595029No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCG770.29811452243602155No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCG760.29424290526152774No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT700.27101320221456504No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT700.27101320221456504No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCG700.27101320221456504No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCG700.27101320221456504No Hit
GAGACTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC650.2516551163420961No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTCG620.24004026481861473No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT540.20906732742266443No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT540.20906732742266443No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT540.20906732742266443No Hit
GAGACTTAGAACAGGCTCCTCTAGGGCTACAGGGGTATCTAATCCCAGTC510.19745247589918308No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG510.19745247589918308No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT500.1935808587246893No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTC500.1935808587246893No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCG480.18583762437570173No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTC470.18196600720120795No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA470.18196600720120795No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTTCT470.18196600720120795No Hit
GAGACTTAGAACAGGCTCCTCTAGCACAGGGGTATCTAATCCCAGTCGCT470.18196600720120795No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCGCTT430.1664795385032328No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTAAT430.1664795385032328No Hit
GAGACTTAGAACAGGCTCCTCTAGCGGCAGGTCAGGGGTATCTAATCCCA430.1664795385032328No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT430.1664795385032328No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTC410.15873630415424522No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT410.15873630415424522No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTT400.15486468697975145No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT390.15099306980525765No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT390.15099306980525765No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGCTCGGGGTATCTAATC380.14712145263076387No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA380.14712145263076387No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGTCGTATGGGGTATCTAATCCCA370.14324983545627007No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCGC360.1393782182817763No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT360.1393782182817763No Hit
GAGACTTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTCGCT350.13550660110728252No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG350.13550660110728252No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAA350.13550660110728252No Hit
GAGACTTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTCGCTT330.12776336675829494No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC330.12776336675829494No Hit
GAGACTTAGAACAGGCTCCTCTATGCGTCAGGGGTATCTAATCCCAGTCG320.12389174958380114No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTC320.12389174958380114No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTC310.12002013240930737No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT310.12002013240930737No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTC300.11614851523481359No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTC290.11227689806031979No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTC290.11227689806031979No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTC280.10840528088582602No Hit
GAGACTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT270.10453366371133221No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAAT260.10066204653683844No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA260.10066204653683844No Hit
GAGACTTAGAACAGGCTCCTCTAGGGCTCGGGGTATCTAATCCCAGTCGC260.10066204653683844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAA25450.0295.05
GAGACTT25550.0294.999971
AGACTTA25550.0294.42272
ACTTAGA25550.0293.84544
TTAGAAC25550.0293.26816
GAACAGG25700.0292.130349
TAGAACA26800.0280.690287
AGAACAG27000.0278.61118
GACTTAG49450.0151.825073
GAACCGA200.006699835147.5295
GAAGGAA200.006699835147.5295
GAGACAT505.8283094E-6118.01
CGAGACG403.571694E-4110.625295
GTTGATT9300.059.00000495-99
GGCGAGC9100.059.00000480-84
GGCGAAT1250.059.00000480-84
GGATATG9300.059.00000425-29
GCCCTCG550.059.025-29
CGTTTGT800.059.060-64
CTACAGG406.1118044E-1059.025-29
Produced by FastQC (version 0.11.5)