FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-116-5-1-3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-116-5-1-3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26046
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC791830.400061429778084No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC276210.604315441910465No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT14685.636182139292021No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATC12714.879827996621362No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC7502.8795208477309373No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCG7082.718267680258005No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC6662.5570145127850727No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4941.8966443983721109No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC3781.4512785072563925No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGAT3511.3476157567380787No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACC3011.1556477002226828No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2851.0942179221377561No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2771.063503033095293No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC2721.0443062274437533No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2651.017430699531598No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACC2651.017430699531598No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCG2510.9636796437072871No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2190.8408200875374339No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1750.6718881978038854No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1570.6027796974583429No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1550.5951009751977271No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1510.5797435306764954No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGA1340.5144743914612608No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTA1320.506795669200645No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1300.4991169469400291No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1130.4338478077247946No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1100.42232972433387084No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1090.4184903632035629No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAA1050.4031329186823312No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGT920.35322122398832834No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC850.3263456960761729No Hit
CGATTCCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA750.28795208477309375No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAA670.2572371957306304No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCG650.24955847347001456No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA630.24187975120939878No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT620.23804039007909086No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT570.21884358442755125No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT560.21500422329724334No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA560.21500422329724334No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACC550.21116486216693542No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAAC540.2073255010366275No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT530.20348613990631959No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAAC530.20348613990631959No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC510.19580741764570375No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTA500.19196805651539584No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTAA500.19196805651539584No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTAACCG500.19196805651539584No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAG490.18812869538508792No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGA480.18428933425478No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTAAC480.18428933425478No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTA450.17277125086385625No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCCGTGGGGTATCTAATCCCAGT450.17277125086385625No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCG410.15741380634262458No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC400.15357444521231667No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGGTATCTAATCCCA390.14973508408200875No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTA390.14973508408200875No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC390.14973508408200875No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC380.14589572295170083No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAG380.14589572295170083No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC370.14205636182139292No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCC360.138217000691085No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAAC360.138217000691085No Hit
CGATTCCTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAAT340.13053827843046917No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT340.13053827843046917No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTGGCTGTCTCTTATACACAGGG330.12669891730016125No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAAC330.12669891730016125No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGCTCGGAGGGGTATCTAATC330.12669891730016125No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAAC330.12669891730016125No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC320.12285955616985335No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT320.12285955616985335No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGATCGGGGTATCTAAT320.12285955616985335No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCCCTCGATCTCCTTGGGGTATCTA310.11902019503954543No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAA310.11902019503954543No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC280.10750211164862167No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCTCTGGGGTATCTAATCCCAGTA280.10750211164862167No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAAC280.10750211164862167No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCG280.10750211164862167No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAAC270.10366275051831375No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTCCAGGTCAGGGGTATCTAATCCC270.10366275051831375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG25700.0295.04
TTCCTAA108.4175915E-4295.04
ATTCCTA25800.0295.03
CGATTCC25950.0294.43161
GATTCCT25900.0294.43052
CCTAGAA25750.0294.42726
TCCTAGA25800.0293.85665
CTAGAAC26150.0289.359477
TAGAACA26900.0280.74358
AGAACAG26900.0280.74359
TCCTAAA150.002833768196.666675
TAAGCGA150.002833768196.66667295
TGAGTAG200.0067001325147.5295
TAGCAAA600.0017889973.75295
ATCCCGT150.00437178459.000004140-144
GGAGATT150.00437178459.000004200-204
CTCTGGT150.00437178459.00000475-79
CGTCAGG150.00437178459.00000425-29
GGGTTTG600.059.00000425-29
GTTTGGG600.059.00000430-34
Produced by FastQC (version 0.11.5)