FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-115-4-1-3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-115-4-1-3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25546
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT446917.4939325138965No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTAA23139.054255069286777No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCTG20868.165661943161357No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT15736.157519768261175No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGAT13925.448993971658968No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTAA12094.732639160729664No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGAT5802.2704141548579035No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATCT5752.250841619040163No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCGA4891.9141940029750253No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGATC4841.894621467157285No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT4591.7967587880685822No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT4191.640178501526658No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCGA3941.5423158224379552No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT3261.2761293353166836No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTAA3051.1939246848821734No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCGA2270.8885931261254209No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTAAC2070.8103029828544587No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTA1980.7750724183825257No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTAA1960.7672434040554293No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTA1950.7633288968918813No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTA1940.7594143897283332No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTAA1820.7124403037657558No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1770.6928677679480154No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA1760.6889532607844673No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAACC1750.6850387536209191No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGAT1710.6693807249667267No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1630.6380646676583418No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAAC1620.6341501604947938No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAAC1290.504971424097706No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCGA1290.504971424097706No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGAT1100.4305957879902921No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1060.4149377593360996No Hit
AGGAATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAACCG870.3405621232286855No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAAC850.3327331089015893No Hit
AGGAAGGACTACCGGGGTATCTAATCCCAGTAACCGATCTGTCTCTTATA820.32098958741094497No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTA810.3170750802473969No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT810.3170750802473969No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT810.3170750802473969No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC810.3170750802473969No Hit
AGGAATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACCG740.2896735301025601No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG740.2896735301025601No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG710.27793000861191575No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACCGAT680.2661864871212714No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACCG660.2583574727941752No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTAACCG630.24661395130353087No Hit
AGGAATAGAACAGGCTCCTCTAGCTCAGATGGTATGAGAGACGGGGTATC600.23487042981288653No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAAC600.23487042981288653No Hit
AGGAATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAACC580.22704141548579035No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGTCGGGGTATCTAATCCCAGTAAC540.21138338683159788No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT530.2074688796680498No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGATC520.2035543725045017No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTAAC520.2035543725045017No Hit
AGGAATAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTAACCGA520.2035543725045017No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTAA510.19963986534095357No Hit
AGGAATAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTAAC500.19572535817740547No Hit
AGGAATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTAACCGA490.19181085101385734No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG440.17223831519611682No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA430.1683238080325687No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT420.1644093008690206No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTAA420.1644093008690206No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACCG410.16049479370547248No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAACCG370.14483676505128004No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG350.13700775072418384No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTAA330.1291787363970876No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAACCG310.12134972206999138No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT290.11352070774289517No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT290.11352070774289517No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT270.10569169341579894No Hit
AGGAATAGAACAGGCTCCTCTAGTCAATGAAATCGTGCGGGGTATCTAAT270.10569169341579894No Hit
AGGAATAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCTAAT270.10569169341579894No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT270.10569169341579894No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACCG260.10177718625225085No Hit
AGGAATAGAACAGGCTCCTCTAGGGTCGGGGTATCTAATCCCAGTAACCG260.10177718625225085No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT260.10177718625225085No Hit
AGGAATAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTAA260.10177718625225085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAG24950.0292.635252
AGGAATA25100.0291.474121
AACAGGC24750.0289.636359
GAACAGG24850.0288.470838
GAATAGA25700.0284.095343
TAGAACA25650.0281.198826
ATAGAAC26000.0279.682685
AGAACAG25950.0277.9487
AATAGAA27000.0269.324074
AGGAATG301.537046E-9245.833311
AACGGCT251.8465653E-7236.09
GAACGGC251.8465653E-7236.08
GGAAGGC202.2561422E-5221.252
GGAATGA353.8653525E-9210.714282
AGGAAGG457.4578566E-11196.666671
AGAACGG304.582016E-7196.666667
AAGGCTC255.493366E-5177.04
GAAGGCT255.493366E-5177.03
GGAAGGA301.13604576E-4147.499982
AAGGACT301.13604576E-4147.499984
Produced by FastQC (version 0.11.5)