FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-114-5-1-3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-114-5-1-3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24894
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT737229.613561500763236No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT279411.223588013175865No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTA20408.19474572186069No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCT13955.603759942154736No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGA7543.0288422913151765No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT7493.0087571302321847No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTA6102.45038965212501No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT5642.265606170161485No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT3731.4983530167911947No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATC3441.3818590825098418No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT3271.3135695348276692No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGAT2621.0524624407487748No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2320.9319514742508235No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGA2290.9199003776010283No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2230.8957981843014381No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA2020.8114405077528721No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2010.8074234755362737No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTA1980.7953723788864787No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCG1940.7793042500200851No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCG1720.6909295412549209No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTA1670.6708443801719289No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1310.5262312203743874No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTAA1230.4940949626416004No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1070.42982244717602636No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT990.39768618944323936No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT960.3856350927934442No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAA950.38161806057684583No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCGA900.3615328994938539No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT860.34546477062746045No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA770.309311480678075No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTAAC770.309311480678075No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT730.29324335181168154No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAA680.27315819072868963No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT550.22093677191291072No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT540.21691973969631237No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAA530.212902707479714No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCG520.20888567526311563No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGA510.20486864304651722No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCG500.20085161082991887No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT500.20085161082991887No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT490.1968345786133205No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTA480.1928175463967221No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTAAC470.18880051418012372No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTA460.18478348196352537No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT460.18478348196352537No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGA430.17273238531373022No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAACC430.17273238531373022No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAAC420.16871535309713184No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT410.16469832088053346No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTA410.16469832088053346No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCGA400.16068128866393508No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGAT400.16068128866393508No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACCGA400.16068128866393508No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT390.15666425644733672No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT390.15666425644733672No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACC370.14863019201413996No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA340.13657909536434482No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTAACCGA330.13256206314774646No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTAAC330.13256206314774646No Hit
GAGACTTAGAACAGGCTCCTCTAGCACATGGGGTATCTAATCCCAGTAAC310.12452799871454968No Hit
GAGACTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACCG300.1205109664979513No Hit
GAGACTTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTAACCG290.11649393428135293No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTAA290.11649393428135293No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT280.11247690206475455No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAGTA280.11247690206475455No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT270.10845986984815618No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACC270.10845986984815618No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT260.10444283763155782No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGACATCGTTTACGGCGTGGACTAC260.10444283763155782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAG108.4153493E-4295.06
GAGACTT24650.0294.40161
AGACTTA24600.0294.400422
ACTTAGA24500.0294.397954
GACTTAG24500.0294.397953
CTTAGAA24450.0294.396735
GAACAGG24450.0293.793469
TTAGAAC24600.0291.402446
TAGAACA25550.0281.14487
AGAACAG25800.0277.848858
GAGACTG150.002833015196.666671
GAAGGCT150.002833015196.666679
TAGAAGG200.0066983565147.57
GAGCGCA200.0066983565147.5295
AGAAGGC200.0066983565147.58
AAACAGG200.0066983565147.59
AGAGGAG200.0066983565147.5295
GACTGAG200.0066983565147.53
GAGACGT200.0066983565147.51
CAGCGAA455.7032966E-498.33333295
Produced by FastQC (version 0.11.5)