FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-113-4-7-5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-113-4-7-5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24389
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC460118.865062118168026No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT277811.390380909426382No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG24099.877403747591128No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC14235.834597564475788No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTT14015.7443929640411655No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT7102.911148468571897No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT5572.2838164746402065No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTT4861.9927016277830172No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCT4731.9393989093443766No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCT4701.9270982820123825No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4391.7999917995817787No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC4151.7015867809258272No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCT3431.4063717249579728No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2991.2259625240887286No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTC2731.1193570872114478No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2631.0783549961048013No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCG2200.9020460043462216No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1950.7995407765796056No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1750.7175365943663127No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1710.7011357579236541No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1540.6314322030423551No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG1490.6109311574890319No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCT1430.586329902825044No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1400.57402927549305No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1350.553528229939727No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGC1280.5248267661650744No Hit
CGATTCCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1220.5002255115010865No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1210.49612530239042196No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC1160.47562425683709864No Hit
CGATTCCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT1020.4182213292877937No Hit
CGATTCCTAGAACAGGCTCCTCTATGCGTCAGGGGTATCTAATCCCAGTC980.40182049284513516No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT940.3854196564024765No Hit
CGATTCCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTT940.3854196564024765No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC790.32391651974250685No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGC780.31981631063184224No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC730.29931526507851897No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGT730.29931526507851897No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGT720.29521505596785436No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTC690.2829144286358604No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTGGGGTATCTAATCCCAGTCGC670.27471401041453114No Hit
CGATTCCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTC620.2542129648612079No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCGCT610.25011275575054326No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA600.24601254663987865No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTC600.24601254663987865No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATC590.24191233752921398No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGTTCGGGGTATCTAATC570.2337119193078847No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTC560.22961171019722004No Hit
CGATTCCTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTCG530.21731108286522613No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC520.2132108737545615No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCT500.2050104555332322No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT490.20091024642256758No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCG490.20091024642256758No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC470.19270982820123825No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTC430.1763089917585797No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGC420.17220878264791506No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTC420.17220878264791506No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGT410.1681085735372504No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC400.16400836442658576No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTT380.15580794620525645No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCGC370.15170773709459182No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCCCCAACAGCTGCACTGGGGTATC360.14760752798392718No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTCGC360.14760752798392718No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA330.13530690065193327No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGCGGGGTATCTAATCCCAGTCG330.13530690065193327No Hit
CGATTCCTAGAACAGGCTCCTCTCGTCGGGGTATCTAATCCCAGTCGCTT310.12710648243060396No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTTC310.12710648243060396No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTT300.12300627331993932No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC280.11480585509861002No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTAATATTATGGGGGTATCTAATCC280.11480585509861002No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA280.11480585509861002No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT280.11480585509861002No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC280.11480585509861002No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCTA270.11070564598794538No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGCGGGGTATCTAATCCC260.10660543687728075No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT260.10660543687728075No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC250.1025052277666161No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCGC250.1025052277666161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA23900.0295.05
GATTCCT24100.0295.02
ATTCCTG108.414308E-4295.03
ATTCCTA24000.0295.03
CCTAGAC157.153827E-6295.06
CCTAGAA23750.0295.06
CGATTCC24300.0295.01
TTCCTAG24000.0294.385444
CTAGAAC23950.0291.30487
AGAACAG25150.0278.578529
TAGAACA25650.0272.57318
CCTGAAC150.0028326656196.666676
TCCAGAA150.0028326656196.666675
TTCCTGA150.0028326656196.666674
TTCCAGA150.0028326656196.666674
GATTCCA304.577796E-7196.666672
ATTCCAA150.0028326656196.666673
TCCTGAA150.0028326656196.666675
CGAGAGG200.006697532147.5295
ATTCCAG200.006697532147.53
Produced by FastQC (version 0.11.5)