FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-112-4-1-3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-112-4-1-3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24663
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC395916.05238616551109No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATC255410.355593399018773No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT24399.889307870088796No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC12755.169687385962778No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT9053.669464379840247No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCG8433.41807565989539No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACC5462.2138425982240606No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT5212.112476178891457No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGAT4881.9786725053724203No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCG4071.650245306734785No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACC3941.597534768681831No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGA3881.5732068280420064No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG3871.569152171268702No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3651.4799497222560112No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA3511.423184527429753No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG3471.4069659003365365No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACC3431.39074727324332No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3391.3745286461501034No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2911.1799051210315046No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTA2721.102866642338726No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAA2681.0866480152455096No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG2010.8149860114341321No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTA1730.7014556217816161No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1670.6771276811417913No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTA1510.6122531727689251No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC1500.6081985159956209No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1440.5838705753557961No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCG1300.5271053805295381No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1230.49872278311640916No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCG1180.47844949924988844No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT1170.47439484247658437No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1150.4662855289299761No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAA1010.40952033410371813No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTA970.3933017070105016No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTAACCGA970.3933017070105016No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCAG940.38113773669058915No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAAC850.3446458257308519No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCG840.34059116895754776No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT830.33653651218424363No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACC750.30409925799781046No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAAC720.29193528767789806No Hit
CCACGTCTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTAACC720.29193528767789806No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTA710.2878806309045939No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC680.2757166605846815No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGA670.2716620038113774No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC610.24733406317155254No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAAC610.24733406317155254No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG590.23922474962494422No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT560.22706077930503182No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGGGTATCTAATCCCAGTAACC520.21084215221181526No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC510.20678749543851113No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGGTATCTAATCCCAGTAA510.20678749543851113No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCTCGGGGGTATCTAATCCCAGT490.19867818189190284No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTA480.1946235251185987No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTA480.1946235251185987No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCA470.19056886834529457No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAAC460.18651421157199044No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTGATGGGGTATCTAATCCCAGTA450.1824595547986863No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTAA450.1824595547986863No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCG440.17840489802538215No Hit
CCACGTCTAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTAA430.17435024125207801No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACC430.17435024125207801No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAAC370.15002230061225316No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTTGGGGTATCTAATCCCAGTA340.13785833029234076No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTAA320.12974901674573247No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC310.12569435997242834No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTCCGGGGTATCTAATCCCAGTAAC300.1216397031991242No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAG290.11758504642582004No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA280.11353038965251591No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC270.10947573287921177No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA270.10947573287921177No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTAACC270.10947573287921177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA108.4149383E-4295.03
CACGCCT108.4149383E-4295.02
GCCTAGA108.4149383E-4295.05
CCACGCT157.1549002E-6294.999971
ACGTCTA24250.0294.391753
CCACGTC24500.0293.795931
CACGTCT24400.0293.7912
CGTCTAG24200.0293.7814
GTCTAGA24300.0292.572025
TCTAGAA24200.0291.952486
CTAGAAC24550.0288.391057
TAGAACA25300.0278.67598
AGAACAG25600.0275.986339
CCTAGAA150.0028328772196.666666
AGAACGG150.0028328772196.666669
CGCCTAG150.0028328772196.666664
CGTCTAA200.006698031147.54
CCACGCC200.006698031147.51
GCTAGAA301.1355878E-4147.499985
CACGCTA352.0981688E-4126.4285662
Produced by FastQC (version 0.11.5)