FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-111-5-7-5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-111-5-7-5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23662
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC751931.776688361085288No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC23199.800524046995182No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG17807.522610092130842No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT13055.515172005747612No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC6912.920294142506973No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC6382.696306313921055No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT5732.4216042599949286No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3521.487617276646099No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC3091.305891302510354No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2621.1072605865945397No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTC2541.0734511030344012No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2290.967796466908968No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCT2100.8874989434536388No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGC2010.8494632744484828No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA1860.7860704927732229No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1790.7564871946581015No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1740.735356267433015No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC1740.735356267433015No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1700.7184515256529457No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTT1670.7057729693178937No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCT1520.6423801876426337No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC1260.5324993660721833No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT1240.5240469951821486No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGC1230.5198208097371313No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGC1120.4733327698419406No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1080.4564280280618714No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1000.4226185445017327No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG990.4183923590567154No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT980.4141661736116981No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT980.4141661736116981No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA900.3803566900515595No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC860.36345194827149013No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC860.36345194827149013No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG820.34654720649142084No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC760.32119009382131686No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA730.3085115374862649No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG680.2873806102611783No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT650.27470205392612623No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC590.2493449412560223No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG530.22398782858591834No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCTTGTGGGGTATCTAATCCCAG530.22398782858591834No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC520.21976164314090105No Hit
TCTCAATCTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGT510.2155354576958837No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCT500.21130927225086635No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCG490.20708308680584905No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC490.20708308680584905No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG480.20285690136083173No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT470.19863071591581438No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGT450.19017834502577974No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC440.1859521595807624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCG400.1690474178006931No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC390.16482123235567575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAG380.16059504691065843No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGCTCTTTCGATCTCGGGGTATCT380.16059504691065843No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTC370.1563688614656411No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT370.1563688614656411No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG360.15214267602062378No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC340.14369030513058914No Hit
TCTCAATCTAGAACAGGCTCCTCTGGACATCGGGGTATCTAATCCCAGTC330.1394641196855718No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGGTATCTAA320.13523793424055447No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCG310.13101174879553715No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTT310.13101174879553715No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA300.12678556335051983No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC290.1225593779055025No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC290.1225593779055025No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC290.1225593779055025No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCTCGTGGGGTATCTAATCCCAG290.1225593779055025No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCT280.11833319246048515No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCG260.10988082157045052No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTCG240.10142845068041587No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGCATCAGGGGTATCTAATCCC240.10142845068041587No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC240.10142845068041587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTC108.412777E-4294.999972
CTCAATC23250.0294.36562
TCTCAAT23450.0293.1131
AATCTAG23250.0293.096775
CTAGAAC23150.0293.088568
TCAATCT23350.0292.473243
TCTAGAA23250.0292.462377
ATCTAGA23350.0291.841556
CAATCTA23550.0289.363074
TAGAACA23950.0282.682659
TCACTCT150.002832151196.666673
TCTCACT150.002832151196.666671
TCTCAAG255.488334E-5177.01
TAGCGAA200.0066963197147.49998295
TCAAGCT200.0066963197147.499983
GCTAGAA200.0066963197147.499987
CTCAAGC200.0066963197147.499982
ACACCGA455.699843E-498.333336295
TTATGGC150.004370808659.000004105-109
GGAGATC150.004370808659.000004200-204
Produced by FastQC (version 0.11.5)