FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-110-5-7-5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-110-5-7-5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23669
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC790933.41501542101483No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC275411.635472559043475No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG17197.262664244370273No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT9704.098187502640585No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC7413.130677257171828No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC6132.5898855042460602No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT4982.104017913726816No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC3631.53365161181292No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGC3411.4407030292788034No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTC3261.377328995732815No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA2851.20410663737378No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG2701.1407326038277916No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC2501.0562338924331405No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2451.0351092145844776No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2330.984409987747687No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2040.8618868562254425No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCT1940.819637500528117No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1880.7942878871097215No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1740.7351387891334658No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1470.6210655287506865No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTT1190.5027673327981749No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1110.46896784824031434No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1080.4562930415311166No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1060.4478431703916515No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT960.4055938146943259No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCT930.39291900798512824No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA930.39291900798512824No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC820.34644471671807003No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT750.31687016772994214No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC700.29574548988127936No Hit
AATCCTATTAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGT690.2915205543115468No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC670.28307068317208167No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTC650.27462081203261646No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCG590.24927119861422112No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC520.2196966496260932No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC510.21547171405636062No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCT470.19857197177743038No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG470.19857197177743038No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTA470.19857197177743038No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC460.19434703620769783No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA450.19012210063796528No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT450.19012210063796528No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTC450.19012210063796528No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCT440.1858971650682327No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCT430.18167222949850015No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC430.18167222949850015No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC410.17322235835903502No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCGGGGGTATCTAATCCCAGTC390.1647724872195699No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC380.16054755164983733No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC380.16054755164983733No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC360.15209768051037223No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA340.1436478093709071No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA340.1436478093709071No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC330.13942287380117455No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG330.13942287380117455No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCT310.1309730026617094No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGT310.1309730026617094No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC300.12674806709197683No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGC300.12674806709197683No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA300.12674806709197683No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG300.12674806709197683No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTT300.12674806709197683No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC290.12252313152224428No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAA290.12252313152224428No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTCG280.11829819595251172No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA280.11829819595251172No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCT270.11407326038277915No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAAGTC270.11407326038277915No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGCGGGGTATCTAATCCC260.1098483248130466No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATC240.10139845367358148No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGC240.10139845367358148No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG240.10139845367358148No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTCTAGGGAGACAGGGGGGTATCT240.10139845367358148No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT240.10139845367358148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT23350.0295.02
CCTATTA23100.0295.04
TCCTATT23200.0295.03
CCTATTG108.412777E-4294.999974
CTTTAGA108.412777E-4294.999975
ATTAGAA22700.0294.350227
AATCCTA23550.0293.747341
TATTAGA23050.0293.080266
CTATTAG23050.0293.080265
TTAGAAC22900.0291.135358
TAGAACA23800.0278.886549
TCCTATA202.2540722E-5221.249983
TCCTTTA150.002832151196.666673
AATCCTT150.002832151196.666671
CCTATAG150.002832151196.666674
CCTTTAG150.002832151196.666674
ATCCTTT150.002832151196.666672
TTAGACA408.569259E-9184.374988
ATTAGAC503.2499884E-8147.57
ATTAAAC352.0970977E-4126.4285667
Produced by FastQC (version 0.11.5)