FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-109-5-1-3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-109-5-1-3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23564
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT747631.72636224749618No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT278411.814632490239347No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTAA17927.604820913257511No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCTG10654.519606178916992No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT7093.0088270242743165No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT5822.469869292140553No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGAT5182.1982685452384993No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT3701.5701918180274994No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTAA2861.2137158377185537No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATCT2671.1330843659820065No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC2461.0439653709047698No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGAT2391.0142590392123578No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT2310.9803089458496012No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT2250.9548463758275335No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCGA2230.9463588524868444No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTAA2150.9124087591240875No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1680.7129519606178917No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTA1670.7087081989475471No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCGA1550.6577830589034119No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1520.6450517738923782No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGATC1460.6195892038703107No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAAC1120.4753013070785944No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1010.4286199287048039No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCGA970.41164488202342553No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG960.407401120353081No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAAC950.4031573586827364No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT930.3946698353420472No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACCGAT880.3734510269903242No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTAA820.34798845696825664No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA700.2970633169241216No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTA600.2546257002206756No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT600.2546257002206756No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTAACC580.24613817687998643No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG560.2376506535392972No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTAA530.22491936852826344No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCGAT520.22067560685791887No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT520.22067560685791887No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT520.22067560685791887No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTAAC500.21218808351722968No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA480.2037005601765405No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTAA480.2037005601765405No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT450.1909692751655067No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCTCGTGGGCTCGGGGTATCTAATCC440.1867255134951621No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTAAC430.18248175182481752No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAACC430.18248175182481752No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTAAC400.16975046681378375No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACCG400.16975046681378375No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCGA390.16550670514343915No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAAC390.16550670514343915No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT370.15701918180274996No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA370.15701918180274996No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTAAC360.15277542013240536No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT360.15277542013240536No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACCG330.14004413512137157No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA320.135800373451027No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGAT300.1273128501103378No Hit
ATCTGTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTAACCGAT300.1273128501103378No Hit
ATCTGTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTAACC290.12306908843999322No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTATTGGGGTATCTAATCCCAGTAA290.12306908843999322No Hit
ATCTGTAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTAACCG280.1188253267696486No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAACCG280.1188253267696486No Hit
ATCTGTAGAACAGGCTCCTCTAGCGAGTCGTATGGGGTATCTAATCCCAG270.11458156509930402No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT260.11033780342895944No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAACCG260.11033780342895944No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACCG260.11033780342895944No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT250.10609404175861484No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCTT250.10609404175861484No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT240.10185028008827025No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT240.10185028008827025No Hit
ATCTGTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCCC240.10185028008827025No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTA240.10185028008827025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG23050.0295.02
ACCACCC108.4125507E-4294.99997295
TCTGTGA108.4125507E-4294.999972
CTGTAGA23150.0293.088563
GAACAGG22800.0293.059238
ATCTGTA23400.0291.84831
AACAGGC22900.0291.135359
AGAACAG23600.0283.125037
GTAGAAC23950.0280.21925
TGTAGAA24200.0279.76244
TAGAACA24100.0277.251046
ATCTGTG304.5745946E-7196.666671
GAAAAGG255.4880395E-5177.08
AAAAGGC200.0066961404147.499989
CTGTGGA200.0066961404147.499983
GTAGAAA403.567767E-4110.624995
GCCCTCG257.790832E-659.00000420-24
CTTTTGA150.004370762559.00000445-49
CTACAGG303.3166907E-759.00000425-29
CGCTGCA150.004370762559.000004175-179
Produced by FastQC (version 0.11.5)