FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-108-5-8-2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-108-5-8-2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23705
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT719730.360683400126554No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT22979.689938831470155No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT17597.420375448217675No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTGT9453.9865007382408773No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTGC7133.007804260704493No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT6962.9360894326091542No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT5342.252689306053575No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT4481.8898966462771567No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC4091.7253743935878505No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT3281.3836743303100612No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTGC2671.1263446530267875No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCTG2260.95338536173803No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT2230.9407298038388526No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTTG2060.869014975743514No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG2010.8479223792448851No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTTG2000.8437038599451592No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1980.8352668213457077No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGCT1750.7382408774520144No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1710.7213668002531112No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG1700.7171482809533853No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG1660.7002742037544821No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTTG1560.6580890107572241No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1490.6285593756591437No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT1230.5188778738662729No Hit
AGGAATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTG1100.46403712296983757No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT980.41341489137312804No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT890.3754482176755959No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTGT880.37122969837587005No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGTG830.35013710187724106No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTTG810.3417000632777895No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTGC780.3290445053786121No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA670.2826407930816283No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTTGC660.27842227378190254No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGTT600.2531111579835478No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT600.2531111579835478No Hit
AGGAATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTGT580.24467411938409617No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGTG580.24467411938409617No Hit
AGGAATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTTGC580.24467411938409617No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT540.22780004218519298No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT520.2193630035857414No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGCT510.2151444842860156No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT500.2109259649862898No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG480.20248892638683821No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG480.20248892638683821No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT470.1982704070871124No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT450.18983336848766083No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT440.18561484918793503No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT430.18139632988820922No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTGTT430.18139632988820922No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA420.17717781058848345No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGTGT420.17717781058848345No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT400.16874077198903184No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGTG400.16874077198903184No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTG380.16030373338958026No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTGTT370.15608521408985446No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGTG370.15608521408985446No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT370.15608521408985446No Hit
AGGAATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGTT360.15186669479012865No Hit
AGGAATAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTGT360.15186669479012865No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA330.13921113689095127No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTTGC330.13921113689095127No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGTG320.1349926175912255No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTGTT310.13077409829149966No Hit
AGGAATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTGC310.13077409829149966No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT310.13077409829149966No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTGGAGGGGTATCTAAT300.1265555789917739No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT290.12233705969204808No Hit
AGGAATAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGTG290.12233705969204808No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT290.12233705969204808No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGTTG280.1181185403923223No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA280.1181185403923223No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG280.1181185403923223No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT270.11390002109259649No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTGTG260.1096815017928707No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG250.1054629824931449No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT250.1054629824931449No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGCAGTCAGGGGTATCTAATCCCAG240.10124446319341911No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT240.10124446319341911No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGC108.412888E-4295.08
GAACAGG23050.0293.080268
AACAGGC23250.0291.827979
GGAATAG23450.0290.597022
AGGAATA23800.0289.422271
ATAGAAC24050.0282.73395
GAATAGA24150.0282.173923
AGAACAG24200.0279.76247
TAGAACA24300.0278.61116
AATAGAA24700.0275.89074
AGTGGAG200.0066964077147.5295
AGAACGG200.0066964077147.57
AACGGCT200.0066964077147.59
GGAATAA403.568051E-4110.6252
GCCCTAT150.004370831459.000004210-214
GCGGGGT150.004370831459.000004125-129
TAGCGGG150.004370831459.000004125-129
CTTCAAA150.004370831459.000004220-224
GGGTTTG900.059.00000425-29
TCCTTAG600.059.00000445-49
Produced by FastQC (version 0.11.5)