FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-107-5-2-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-107-5-2-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23086
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT741932.13635969851858No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT246110.660140344797712No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG17287.485055878021312No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT11625.033353547604609No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT8713.7728493459239365No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT7023.0408039504461577No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC4371.8929221173005284No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT3471.5030754569869185No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG2981.290825608593953No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG2911.2605042016806722No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT2491.0785757602009876No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2240.9702850212249848No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTT2230.9659533916659447No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC2170.939963614311704No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT2170.939963614311704No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1610.6973923590054579No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC1560.6757342112102573No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT1550.6714025816512172No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTTG1310.5674434722342545No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1270.5501169539980941No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGGC1090.4721476219353721No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGGC1010.4374945854630512No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1010.4374945854630512No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTTG980.42449969678593086No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT930.4028415489907304No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTTG910.3941782898726501No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG850.3681885125184094No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTT800.34653036472320886No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG720.311877328250888No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGTG660.2858875508966473No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC640.2772242917785671No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTGT640.2772242917785671No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT640.2772242917785671No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT620.26856103266048686No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT600.2598977735424066No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTGT570.2469028848652863No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTT570.2469028848652863No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGCC540.233907996188166No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG530.2295763666291259No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA530.2295763666291259No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTTGT480.20791821883392533No Hit
ATCTGTAGAACAGGCTCCTCTAGGCACTCGATCTCCTTGGGGTATCTAAT480.20791821883392533No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGTGG470.20358658927488524No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGCTCGGGGTATCTAATCCCA470.20358658927488524No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTGT470.20358658927488524No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTTGTGG440.19059170059776487No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT430.18626007103872477No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTTGTG420.18192844147968465No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT400.17326518236160443No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTGG380.1646019232435242No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT360.155938664125444No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT320.13861214588928356No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTT320.13861214588928356No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTTG310.13428051633024343No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTTGTGG300.1299488867712033No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT300.1299488867712033No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTT290.1256172572121632No Hit
ATCTGTAGAACAGGCTCCTCTAGAGCGTCAGGGGTATCTAATCCCAGTTG290.1256172572121632No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTT290.1256172572121632No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT280.1212856276531231No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGCC280.1212856276531231No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTTGTGG270.116953998094083No Hit
ATCTGTAGAACAGGCTCCTCTAGCTCTCTGTGGGGGTATCTAATCCCAGT270.116953998094083No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT270.116953998094083No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGCC270.116953998094083No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGGC250.10829073897600278No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG250.10829073897600278No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCATTGGGGTATCTAAT250.10829073897600278No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTGTGG250.10829073897600278No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT240.10395910941696267No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT240.10395910941696267No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTTG240.10395910941696267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTA23050.0293.080261
TCTGTAG23000.0293.076082
GAACAGG22800.0292.41238
CTGTAGA23100.0291.807373
AACAGGC22800.0291.765359
TGTAGAA23350.0287.41974
GTAGAAC23550.0284.978765
AGAACAG23600.0283.1257
TAGAACA23700.0282.552736
GACAGGC150.0028316854196.666678
CACCGAA200.0066952207147.5295
TCGAAAA200.0066952207147.5295
ATCCCGG150.00437052759.000004200-204
GGATGTG150.00437052759.00000425-29
ACCGGGG303.316054E-759.00000425-29
TTGTTGA8600.059.00000490-94
ACTGCAT150.00437052759.000004215-219
TGGTTAG150.00437052759.000004195-199
TCCTTAG900.059.00000445-49
GTTTGGG950.059.00000425-29
Produced by FastQC (version 0.11.5)