FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-106-6-7-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-106-6-7-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22483
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1174652.243917626651246No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT19778.793310501267625No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT11725.212827469643731No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT5352.379575679402215No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT5242.330649824311702No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT4461.983721033669884No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT3641.6190010229951517No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1940.8628741715963173No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1590.7072009963083219No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGAATATAAAGCACCGCCAAGTCCT1290.5737668460614687No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT1110.49370635591335676No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT990.44033269581461554No Hit
GAGTGGTAGAACAGGCTCCTCTCGGGCGGTTTGGCCAAAAACGCTGGTGA950.42254147578170176No Hit
GAGTGGTAGAACAGGCTCCTCTAGCCAGAATCTTGTAAAAAAATCTGCGC920.40919806075701637No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGTATATGAAGCACCGCCAGGTCCT860.3825112307076458No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCCT830.3691678156829605No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCTCCAGGTCCT820.36472001067473203No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA810.3602722056665036No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAAGTTTC750.3335853756171329No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTATGAGGCACCGCCAAGTCCT730.32468976560067603No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCCT720.32024196059244764No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT690.3068985455677623No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTTCT680.30245074055953386No Hit
GAGTGGTAGAACAGGCTCCTCTGGTGTTGTGCGGGATTATCGTTCGGCTA640.28465952052662014No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCCT590.2624204954854779No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT570.253524885469021No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT530.2357336654361073No Hit
GAGTGGTAGAACAGGCTCCTCTCCTTTCAAAGAGGATGCAGTATGGTTTC480.2134946403949651No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCAT470.20904683538673663No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT420.18680781034559446No Hit
GAGTGGTAGAACAGGCTCCTCTTCACCAACCGTTTCAACCTCTTTGGAGA410.18236000533736602No Hit
GAGTGGTAGAACAGGCTCCTCTACTTGTGCGGGTCCCCGTCTATTCCTTT370.16456878530445224No Hit
GAGTGGTAGAACAGGCTCCTCGCAGTTGTCCTCAGAGCGATTTACTGCCC330.14677756527153848No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC330.14677756527153848No Hit
GAGTGGTAGAACAGGCTCCTCTAGCCAGAATCTAGTAAAAAAACTCTGCG320.14232976026331007No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT320.14232976026331007No Hit
GAGTGGTAGAACAGGCTCCTCTGGGATATCGTAGTAATTTCAGATTGGTT290.12898634523862473No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGACCT280.1245385402303963No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTTAGATACGGCGGGGTTCATAGATT270.12009073522216787No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT250.111195125205711No Hit
GAGTGGTAGAACAGGCTCCTCTGGTCGTTTGTAAAGCCGCCTATTCTATT250.111195125205711No Hit
GAGTGGTAGAACAGGCTCCTCTACACGGGTCCCGAAGGAAAACTCTATCT240.10674732019748255No Hit
GAGTGGTAGAACAGGCTCCTCTCGGGAGGTTTGGCCAAAAGCGCTGGTGA240.10674732019748255No Hit
GAGTGGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT240.10674732019748255No Hit
GAGTGGTAGAACAGGCTCCTCTCGGGAGGTTTGGCCAAAAACGCTGGTGA240.10674732019748255No Hit
GAGTGGTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT230.10229951518925409No Hit
GAGTGGTAGAACAGGCTCCTCTCATACGCAGTTACTGGCAACTGCATTAT230.10229951518925409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC108.4099284E-4295.05
TGGTAAA157.1463746E-6295.04
GGTAGAA21600.0295.05
GTGGCAG157.1463746E-6295.03
GGCAGAA157.1463746E-6295.05
AGTGGTA22000.0293.65912
TGGTAGA21750.0293.643684
GAGTGGT22050.0292.99321
GTGGTAG21950.0291.640083
GAACAGG21650.0291.593549
GTAGAAC22200.0285.69826
AGAACAG22500.0282.544438
TAGAACA22950.0275.076237
TGGCAGA202.2525472E-5221.254
GTGGTAA202.2525472E-5221.253
CAATGAG150.0028311948196.66667295
GCAAGCG150.0028311948196.66667295
AGTGGAG150.0028311948196.666672
GCTACAG150.0028311948196.66667295
CTTCCTC150.0028311948196.66667295
Produced by FastQC (version 0.11.5)