FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-105-5-2-6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-105-5-2-6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22877
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT749732.77090527604144No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT250310.941119902085065No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG15876.937098395768676No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT10394.5416794160073435No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT7773.3964243563404293No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC5662.47410062508196No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT5562.4303885999038335No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT4301.8796170826594394No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG3531.5430344887878655No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT2971.2982471477903572No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT2811.2283079075053547No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2801.223936704987542No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG2761.2064518949162915No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC2471.0796870218997245No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT1670.7299908204747126No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTT1580.6906499978143987No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1550.6775363902609608No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTTG1360.5944835424225204No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC1310.5726275298334572No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTT1290.5638851247978318No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT1210.5289155046553307No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT1110.48520347947720416No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1030.450233859334703No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTT890.38903702408532587No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG830.36280980897845No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGTG800.349696201425012No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGGCC750.3278401888359488No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT740.32346898631813614No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC660.28849936617563493No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTTG650.2841281636578223No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTTGT520.2273025309262578No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT480.20981772085500722No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT470.20544651833719457No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT450.19670411330156928No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGGC440.1923329107837566No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGGC440.1923329107837566No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC420.18359050574813132No Hit
AGGAATAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAGTT410.17921930323031868No Hit
AGGAATAGAACAGGCTCCTCTAGTCCCGTGGGGTATCTAATCCCAGTTGT400.174848100712506No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA390.17047689819469336No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT390.17047689819469336No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT380.16610569567688072No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG370.16173449315906807No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT370.16173449315906807No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTT370.16173449315906807No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGGC360.1573632906412554No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA360.1573632906412554No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT360.1573632906412554No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGCC360.1573632906412554No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTTGTG350.15299208812344275No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTTGTG350.15299208812344275No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTTG350.15299208812344275No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGCC330.14424968308781747No Hit
AGGAATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGCC330.14424968308781747No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAGT330.14424968308781747No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTT320.1398784805700048No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGCC300.1311360755343795No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTGT300.1311360755343795No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTTG290.12676487301656686No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTTGT280.1223936704987542No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT280.1223936704987542No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGCC270.11802246798094154No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT270.11802246798094154No Hit
AGGAATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT260.1136512654631289No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT230.10053765790969096No Hit
AGGAATAGAACAGGCTCCTCTAGCTAAGCTTGGAACAGGGCGGGGGGTAT230.10053765790969096No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTTG230.10053765790969096No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGC108.4109115E-4295.08
AGGAATA22450.0293.685971
GGAATAG22400.0293.024542
GAATAGA22650.0289.79033
GAACAGG22300.0289.04718
AACAGGC22300.0289.04719
ATAGAAC23350.0278.576025
AGAACAG23250.0278.505377
TAGAACA23300.0277.90776
AATAGAA24150.0269.95864
AGGAATG202.2530734E-5221.251
GACAGGC150.0028315245196.666678
AGAACGG150.0028315245196.666677
AACGGCT150.0028315245196.666679
GGAATGA255.4859083E-5177.02
AACAGCT200.006694842147.59
AATGAAC403.5663875E-4110.6254
TGAACAG508.6603605E-488.56
GAATGAA600.001786194373.753
GCCCTCG452.5465852E-1159.00000420-24
Produced by FastQC (version 0.11.5)