FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-104-4-5-7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-104-4-5-7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22882
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG360315.746001223669259No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC22379.776243335372783No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAG21779.514028494012761No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATC14916.516038807796522No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG7243.164059085744253No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC6402.796958307840224No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCAT6132.678961629228214No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT6062.648369897736212No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG5852.5565947032602043No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCA5762.5172624770562013No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATC3871.6912857267721353No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCC3501.5295865746001223No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3501.5295865746001223No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3401.4858841010401187No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG2841.2411502491040993No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCAT2731.1930775281880954No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTC2591.1318940652040905No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCA2120.9264924394720742No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCAT1880.8216065029280658No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC1770.7735337820120619No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC1660.725461061096058No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1490.6511668560440521No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATC1450.6336858666200507No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1390.6074643824840487No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1370.598723887772048No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG1280.5593916615680448No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCAT1180.5156891880080412No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC990.43265448824403463No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG960.41954374617603357No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC950.41517349882003324No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATC850.3714710252600297No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA840.36710077790402934No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT830.362730530548029No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC750.3277685517000262No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC740.32339830434402583No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCA740.32339830434402583No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCAT710.31028756227602483No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT690.30154706756402416No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG680.2971768202080238No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGTCAGGGGTATCTAATCCCAG670.29280657285202344No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG670.29280657285202344No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATC670.29280657285202344No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGCACATGGGGTATCTAATCCCA630.27532558342802205No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGAGTGGGGGTATCTAATCCCAG620.27095533607202166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATC620.27095533607202166No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC600.26221484136002093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATC590.2578445940040206No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA580.25347434664802027No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGT560.24473385193601957No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGCGGGGTATCTAATCCCAGT550.24036360458001924No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA550.24036360458001924No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCA540.23599335722401887No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA530.23162310986801854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCCGTGGGGGTATCTAATCCCA530.23162310986801854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCAT530.23162310986801854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCA500.21851236780001748No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT490.21414212044401715No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT490.21414212044401715No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCC470.20540162573201645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATC470.20540162573201645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA470.20540162573201645No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT450.19666113102001573No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTGGGGTATCTAATCCCAG430.18792063630801503No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCA390.17043964688401364No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG370.16169915217201292No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCC370.16169915217201292No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT370.16169915217201292No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTCAT360.15732890481601258No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTC330.14421816274801155No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCAGATGGTATAAGAGACGGGGT330.14421816274801155No Hit
TTCTCAGCTAGAACAGGCTCCTCTATTCGTGGGGGTATCTAATCCCAGTC330.14421816274801155No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCA330.14421816274801155No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAG320.1398479153920112No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAG310.13547766803601083No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCCGGGGTATCTAATCCCAGTCAT300.13110742068001047No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC280.12236692596800978No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTC270.11799667861200944No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC250.10925618390000874No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGCGGGGGTATCTAATCCCAGTCA240.10488593654400839No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGT240.10488593654400839No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGT240.10488593654400839No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTAGGGGTATCTAATCCCAGTCA230.10051568918800803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA230.10051568918800803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC230.10051568918800803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCATCC230.10051568918800803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT230.10051568918800803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAGC22350.0295.02
TTCTCAG22400.0295.01
CTCAGCT22350.0293.680083
TCAGCTA22450.0292.371954
CAGCTAG22350.0292.360175
GCTAGAA22350.0291.040257
AGCTAGA22550.0289.767186
CTAGAAC22750.0287.219768
TAGAACA23950.0272.212959
TTCTCAT202.2530734E-5221.251
CGTGCAG200.006694842147.5295
TCACTAG200.006694842147.54
TTCTCAC200.006694842147.51
CTTGTGG150.0043704359.00000425-29
CCCCTGG150.0043704359.00000475-79
AGTCCCC150.0043704359.00000475-79
TCTCTTC150.0043704359.000004160-164
GTCTGGC150.0043704359.00000480-84
GGGCTCG303.3157994E-759.00000430-34
ACGTTCT150.0043704359.000004190-194
Produced by FastQC (version 0.11.5)