FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-103-4-4-1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-103-4-4-1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22710
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG300013.210039630118892No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC295913.0295024218406No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT252911.136063408190225No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC14066.191105239982387No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC8273.6415675913694407No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG7573.3333333333333335No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTAC5092.2413033905768383No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA4632.0387494495816822No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG4331.9066490532804932No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3811.677675033025099No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC3701.6292382210479965No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACC3581.5763980625275211No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA2941.2945838837516512No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC2821.2417437252311756No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTA2761.215323645970938No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT2681.180096873623954No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC2451.078819903126376No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT2210.973139586085425No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTA1700.7485689123734038No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGT1680.7397622192866579No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT1670.7353588727432848No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT1660.7309555261999119No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCC1510.6649053280493175No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTA1500.6605019815059445No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT1470.6472919418758256No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG1470.6472919418758256No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG1210.5328049317481286No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTAC1180.5195948921180097No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1020.44914134742404227No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC990.4359313077939234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT990.4359313077939234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTAC880.38749449581682077No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA880.38749449581682077No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA870.3830911492734478No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT860.37868780273007485No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC850.3742844561867019No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA850.3742844561867019No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC820.361074416556583No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTA810.3566710700132101No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG810.3566710700132101No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGCGTCTGGGGTATCTAATCCCA770.3390576838397182No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTAC740.3258476442095993No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTAC740.3258476442095993No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT740.3258476442095993No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT740.3258476442095993No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG710.31263760457948037No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA680.2994275649493615No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGT670.2950242184059885No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT670.2950242184059885No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC660.2906208718626156No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTG620.27300748568912375No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAG620.27300748568912375No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA600.26420079260237783No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG580.25539409951563186No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA570.2509907529722589No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC520.2289740202553941No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAAT490.2157639806252752No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTG470.2069572875385293No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC450.19815059445178335No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGCGTCAGGGGTATCTAATCCCAG420.18494055482166447No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC410.1805372082782915No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTA410.1805372082782915No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTA380.1673271686481726No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTAC380.1673271686481726No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTCGGGGGTATCTAATCCCAGT380.1673271686481726No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTAGGCTCGGGGTATCTAA370.16292382210479964No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG360.15852047556142668No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC350.15411712901805372No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTG340.14971378247468076No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTGT340.14971378247468076No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCCGTGCGGGGTATCTAATCCCA330.1453104359313078No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC300.13210039630118892No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCTGGGGGTATCTAATCCCAGT290.12769704975781593No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGGTATCTAATCCCAGTGTA280.12329370321444298No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTGTAC270.11889035667107001No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC270.11889035667107001No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTATCCCAGT250.11008366358432409No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT240.10568031704095111No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGT230.10127697049757817No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGT230.10127697049757817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCAG22450.0295.000031
TCTCAGC22350.0295.02
CTCAGCT22250.0295.03
TCGCTAG108.410548E-4294.999974
TCTCGCT108.410548E-4294.999972
CTCGCTA108.410548E-4294.999973
TTCTCGC108.410548E-4294.999971
TCAGCTA22300.0293.677124
GCTAGAA22100.0292.997747
CAGCTAG22350.0291.700235
AGCTAGA22300.0291.69286
CTAGAAC22150.0290.33868
TAGAACA23450.0274.243079
CGCTAGA150.0028314025196.666675
GCTAGAC255.4854332E-5177.07
TCTCAGA200.006694554147.499982
TATTTCT452.5465852E-1159.000004100-104
TCTGAGG900.059.00000430-34
GGGTCTG900.059.00000425-29
TTTGTTG3550.059.00000490-94
Produced by FastQC (version 0.11.5)