FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-102-5-4-1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-102-5-4-1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22036
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC513323.293701216191685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG222610.101651842439646No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT18228.268288255581774No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC15507.033944454528952No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC9794.442730078054093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG5222.3688509711381376No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC4532.055726992194591No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4141.8787438736612816No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTAC3901.7698311853330915No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA3711.6836086404066073No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG3221.4612452350698857No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA3051.3840987475040842No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC2641.1980395716100927No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2461.1163550553639499No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACC2341.0618987111998548No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTA2050.9302958794699582No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1880.8531493919041568No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1760.7986930477400617No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1750.7941550190597205No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1600.7260845888546016No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1470.6670902160101652No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1370.6217099292067526No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGT1260.5717916137229987No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTA1210.5491014703212924No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT1110.5037211835178799No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGT990.44926483935378475No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCC900.40842258123071334No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTA870.3948084951896896No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG870.3948084951896896No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTAC840.3811944091486658No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT820.3721183517879833No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG790.35850426574695954No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC780.35396623706661823No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTAC780.35396623706661823No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT770.34942820838627703No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA660.29950989290252317No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC660.29950989290252317No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC630.28589580686149935No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT630.28589580686149935No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA620.2813577781811581No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTA610.27681974950081684No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTAC600.2722817208204756No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG600.2722817208204756No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTAC580.26320566345979307No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGCGTCTGGGGTATCTAATCCCA560.25412960609911056No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC540.24505354873842802No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGT520.23597749137774549No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC500.22690143401706298No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT480.2178253766563805No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA470.2132873479760392No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTA460.20874931929569795No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT460.20874931929569795No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAAT430.19513523325467416No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT430.19513523325467416No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC410.18605917589399165No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA390.17698311853330911No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTG390.17698311853330911No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAG390.17698311853330911No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC380.1724450898529679No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC370.1679070611726266No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG360.16336903249228535No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGCGTCAGGGGTATCTAATCCCAG350.1588310038119441No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC330.14975494645126158No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTG320.14521691777092033No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC320.14521691777092033No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTG320.14521691777092033No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC310.14067888909057905No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTA300.1361408604102378No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGGGTATCTAATCCCAGTGTAC300.1361408604102378No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC300.1361408604102378No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG290.13160283172989654No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTAC280.12706480304955528No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTGT280.12706480304955528No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCCGTGCGGGGTATCTAATCCCA270.12252677436921401No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTCGGGGGTATCTAATCCCAGT250.11345071700853149No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTA250.11345071700853149No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC240.10891268832819025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCTA21750.0294.999974
TTCTCAG21950.0294.328031
CAGCTAG21600.0294.317145
TCTCAGC21950.0293.656042
CTCAGCT21900.0293.652983
AGCTAGA21750.0292.287356
CTAGAAC21800.0291.616948
GCTAGAA21750.0291.60927
TAGAACA22450.0281.859689
CAGCTAC200.0066931555147.499985
GCTAAAC200.0066931555147.499987
ACTGTTA257.787434E-659.000004105-109
GTTTGTG452.5465852E-1159.00000465-69
GGGTCTG1800.059.00000425-29
GAGCGGT452.5465852E-1159.00000485-89
GCGGTTT452.5465852E-1159.00000485-89
TCGGTAA452.5465852E-1159.00000495-99
TTTTCTA650.059.000004100-104
CCGCCAA2750.059.00000440-44
AGGGTTT257.787434E-659.000004110-114
Produced by FastQC (version 0.11.5)