FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-101-4-7-5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-101-4-7-5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22201
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT430219.37750551776947No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC236510.652673303004368No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGT19158.625737579388316No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT13846.233953425521372No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTC7353.3106616819062205No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTG5622.5314175037160487No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTT5292.3827755506508717No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT4351.959371199495518No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT4161.8737894689428405No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTC4141.864780865726769No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC3751.6891131030133777No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGC3621.630557182108914No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCT3471.5629926579883788No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2721.2251700373857033No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC2491.1215711004008828No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2060.9278861312553489No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1950.8783388135669564No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1900.855817305526778No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCG1880.8468087023107067No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTT1730.7792441781901716No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCT1540.6936624476374939No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1520.6846538444214224No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTCG1510.6801495428133868No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCT1430.6441151299491014No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1390.6260979235169587No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTC1290.5810549074366019No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTT1280.5765506058285663No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1220.5495247961803522No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTC1180.5315075897482096No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTC970.4369172559794604No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC950.427908652763389No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTC920.41439574793928197No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCG900.4053871447232107No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCGCTT870.39187423989910364No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA810.3648484302508896No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCG800.3603441286428539No Hit
TGCTTATAGAACAGGCTCCTCTAGGATTATGGGGTATCTAATCCCAGTCG790.35583982703481826No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT780.3513355254267826No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC760.3423269222107112No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCT750.33782262060267554No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTCG750.33782262060267554No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT740.3333183189946399No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACTGGGGTATCTAATCCCAGTCGC700.31530111256249715No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTC700.31530111256249715No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCGC680.3062925093464258No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCG670.30178820773839016No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCGCTTC630.28377100130624744No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT590.2657537948741048No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT580.26124949326606905No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTCG580.26124949326606905No Hit
TGCTTATAGAACAGGCTCCTCTAGTATCCATCGTTTACGGCTAGGACTAC550.24773658844196209No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGCGGCACGCAGGGGTATCTAATCC500.22521508040178373No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTC490.22071077879374804No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTC460.20719787396964098No Hit
TGCTTATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTC440.19818927075356965No Hit
TGCTTATAGAACAGGCTCCTCTAGGTAGGGGTATCTAATCCCAGTCGCTT440.19818927075356965No Hit
TGCTTATAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTCG420.18918066753749832No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTC400.18017206432142696No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTCGCTTCT400.18017206432142696No Hit
TGCTTATAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTC400.18017206432142696No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCAG380.1711634611053556No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTC370.16665915949731994No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTCGCTTC360.16215485788928427No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGTTGGGGTATCTAATCCCAGTCG360.16215485788928427No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCGC350.15765055628124858No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG340.1531462546732129No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCT330.14864195306517725No Hit
TGCTTATAGAACAGGCTCCTCTAAGACTACTGGGGTATCTAATCCCAGTC330.14864195306517725No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGCT320.14413765145714158No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTCGCT320.14413765145714158No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGATTGGGGTATCTAATCCCAGTCG310.13963334984910591No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC300.13512904824107022No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGTGTCCGGGGTATCTAATCCCAGT290.13062474663303453No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTCGC290.13062474663303453No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCGCT280.12612044502499886No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT270.1216161434169632No Hit
TGCTTATAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCC260.11711184180892753No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT260.11711184180892753No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTCGCTT260.11711184180892753No Hit
TGCTTATAGAACAGGCTCCTCTACTGGGGGTATCTAATCCCAGTCGCTTC260.11711184180892753No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTGAAATGGGGAAATAGGGGAGGGG250.11260754020089186No Hit
TGCTTATAGAACAGGCTCCTCTAGCTGGCTCAGGTCGGGTTTAGAGCGGG250.11260754020089186No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT250.11260754020089186No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTC250.11260754020089186No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA240.10810323859285617No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTTGGGGTATCTAATCCCAGTCGC240.10810323859285617No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT230.10359893698482049No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCGCT230.10359893698482049No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTCTCGGGGGTATCTAATCCCAG230.10359893698482049No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT230.10359893698482049No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA108.409296E-4295.03
TGCTTAT21950.0295.01
CTTTAGA108.409296E-4295.03
GCTTTAG108.409296E-4295.02
CTTATAG22000.0293.65913
GCTTATA22150.0292.336332
TTATAGA22150.0291.004524
GAACAGG21800.0290.940379
TATAGAA22750.0282.0335
ATAGAAC22800.0280.120646
TAGAACA22950.0278.289767
AGAACAG23450.0271.727088
TGCTTTA150.0028309827196.666671
TTTAGAA200.006693563147.54
TGCTTAA200.006693563147.51
GCTTAAG200.006693563147.52
CAGGTCC4800.059.00000440-44
TGGAGTT150.00437010359.000004105-109
CGGCGTA150.00437010359.000004210-214
GGGGAAT150.00437010359.00000480-84
Produced by FastQC (version 0.11.5)