FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-100-5-5-7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-100-5-5-7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21856
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC636129.10413616398243No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT19769.04099560761347No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC18698.551427525622254No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC13696.263726207906297No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC6352.905380673499268No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5482.507320644216691No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC5192.3746339677891655No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3591.6425695461200585No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC3441.5739385065885798No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3381.5464860907759883No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC2711.2399341142020497No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT2511.1484260614934114No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC2211.011163982430454No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC2150.9837115666178623No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG2060.9425329428989752No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC2000.9150805270863837No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1820.8327232796486091No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1720.7869692532942899No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCC1680.7686676427525623No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCA1600.7320644216691069No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1570.7183382137628112No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCA1450.6634333821376281No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1170.5353221083455344No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG1130.5170204978038068No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1000.4575402635431918No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC920.42093704245973645No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC830.3797584187408492No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG780.35688140556368964No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC720.32942898975109813No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCA700.32027818448023426No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCAT690.3157027818448023No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC680.31112737920937045No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC670.3065519765739385No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCAT640.29282576866764276No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC620.28367496339677895No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA610.279099560761347No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT520.23792093704245976No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC500.2287701317715959No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT500.2287701317715959No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCA500.2287701317715959No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCAT500.2287701317715959No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC460.21046852122986823No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCAT440.20131771595900438No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGTGCGGGGGTATCTAATCCCAGTC430.19674231332357248No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC420.19216691068814057No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA420.19216691068814057No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCATCC420.19216691068814057No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG400.18301610541727673No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC400.18301610541727673No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC390.17844070278184482No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAGT390.17844070278184482No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATC370.16928989751098097No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT370.16928989751098097No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA360.16471449487554907No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCA360.16471449487554907No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGCTACTCGGGGTATCTAATCCCAG350.16013909224011713No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG350.16013909224011713No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC340.15556368960468522No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCC340.15556368960468522No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT330.1509882869692533No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTC320.14641288433382138No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATC320.14641288433382138No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT310.14183748169838947No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT300.13726207906295754No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCA290.13268667642752563No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCC280.1281112737920937No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGAAATAACAGGGGTATCTAATCCCA280.1281112737920937No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGT280.1281112737920937No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT270.12353587115666179No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC270.12353587115666179No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTC260.11896046852122988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC240.10980966325036604No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGCGGGGTATCTAATCCCAGTCAT240.10980966325036604No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCCAGTTCGAATCGCGATTGTCGG240.10980966325036604No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCC240.10980966325036604No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAGGTCC230.10523426061493411No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTC220.10065885797950219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT21800.0294.32341
GTTAGAA21500.0294.313966
CGAAGTT21850.0293.64992
TTAGAAC21500.0293.627937
AAGTTAG21750.0292.287354
AGTTAGA21750.0292.287355
GAAGTTA21900.0291.632423
TAGAACA22700.0278.75558
AGAACAG22750.0278.142859
GATCGAA150.0028306777196.66666295
GCCACGA150.0028306777196.66666295
GAAAGGA150.0028306777196.66666295
CACGCAA200.006692844147.5295
AGAACGG200.006692844147.5295
CGTTTGT257.787077E-659.00000460-64
CAAGATT257.787077E-659.000004110-114
GAGGCAC2600.059.00000430-34
GTTTGTG257.787077E-659.00000465-69
TTGTTGA7100.059.00000490-94
GGGTCTG2600.059.00000425-29
Produced by FastQC (version 0.11.5)